几种植物基因的信使核糖核酸转录本在体内的多个位点进行多聚腺苷酸化。
mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo.
作者信息
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J
出版信息
Nucleic Acids Res. 1986 Mar 11;14(5):2229-40. doi: 10.1093/nar/14.5.2229.
Abstract
We have analyzed the polyadenylation sites for the small subunit of ribulose bisphosphate carboxylase and chlorophyll a/b binding protein genes of Petunia (Mitchell) and the bronze gene of Zea mays. Sequence analysis of multiple cDNA clones revealed that polyadenylation of the transcripts occurred at either 2 or 3 sites for all three groups of genes. In the examples where 3 polyadenylation sites were detected, the middle site was the one predominantly used. Putative polyadenylation signals preceding the poly A tails diverged significantly from the animal consensus sequence AATAAA. In all the genes examined the first A residue in the poly A tail of the cDNA clones corresponded to an A residue in the homologous genomic sequence.
摘要
我们分析了矮牵牛（Mitchell）的1,5-二磷酸核酮糖羧化酶小亚基和叶绿素a/b结合蛋白基因以及玉米青铜基因的聚腺苷酸化位点。对多个cDNA克隆的序列分析表明，所有这三组基因的转录本的聚腺苷酸化发生在2个或3个位点。在检测到3个聚腺苷酸化位点的例子中，中间位点是主要使用的位点。聚腺苷酸尾巴之前的推定聚腺苷酸化信号与动物共有序列AATAAA有显著差异。在所有检测的基因中，cDNA克隆的聚腺苷酸尾巴中的第一个A残基与同源基因组序列中的一个A残基相对应。
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