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1
mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo.几种植物基因的信使核糖核酸转录本在体内的多个位点进行多聚腺苷酸化。
Nucleic Acids Res. 1986 Mar 11;14(5):2229-40. doi: 10.1093/nar/14.5.2229.
2
Comparison of the expression of two highly homologous members of the soybean ribulose-1,5-bisphosphate carboxylase small subunit gene family.大豆核酮糖-1,5-二磷酸羧化酶小亚基基因家族两个高度同源成员的表达比较
Plant Mol Biol. 1990 Jun;14(6):909-25. doi: 10.1007/BF00019389.
3
Deletion analysis of the polyadenylation signal of a pea ribulose-1,5-bisphosphate carboxylase small-subunit gene.豌豆核酮糖-1,5-二磷酸羧化酶小亚基基因多聚腺苷酸化信号的缺失分析
Plant Mol Biol. 1989 Aug;13(2):125-38. doi: 10.1007/BF00016132.
4
Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose 1,5-bisphosphate carboxylase and the major chlorophyll a/b-binding thylakoid polypeptide.编码1,5-二磷酸核酮糖羧化酶小亚基和主要叶绿素a/b结合类囊体多肽的两个豌豆cDNA克隆的核苷酸序列。
J Biol Chem. 1983 Feb 10;258(3):1399-402.
5
Identification and characterization of cryptic polyadenylation sites in the 3' region of a pea ribulose-1,5-bisphosphate carboxylase small subunit gene.豌豆核酮糖-1,5-二磷酸羧化酶小亚基基因3'区域中隐蔽多聚腺苷酸化位点的鉴定与表征
DNA. 1988 Jun;7(5):329-36. doi: 10.1089/dna.1.1988.7.329.
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Polyadenylation occurs at multiple sites in maize mitochondrial cox2 mRNA and is independent of editing status.聚腺苷酸化作用发生在玉米线粒体cox2 mRNA的多个位点，且与编辑状态无关。
Plant Cell. 1999 Aug;11(8):1565-78. doi: 10.1105/tpc.11.8.1565.
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Upstream sequences other than AAUAAA are required for efficient messenger RNA 3'-end formation in plants.除AAUAAA之外的上游序列对于植物中有效的信使核糖核酸3'末端形成是必需的。
Plant Cell. 1990 Dec;2(12):1261-72. doi: 10.1105/tpc.2.12.1261.
8
Evolution of RLSB, a nuclear-encoded S1 domain RNA binding protein associated with post-transcriptional regulation of plastid-encoded rbcL mRNA in vascular plants.RLSB的进化，一种与维管植物中质体编码的rbcL mRNA转录后调控相关的核编码S1结构域RNA结合蛋白。
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Confirmation of the relative expression levels of the Petunia (Mitchell) rbcS genes.矮牵牛（米切尔）核酮糖-1,5-二磷酸羧化酶小亚基基因相对表达水平的确认。
Nucleic Acids Res. 1987 Jun 11;15(11):4655-68. doi: 10.1093/nar/15.11.4655.
10
Complete sequence of one of the mRNAs coding for the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris.野生烟草核酮糖二磷酸羧化酶小亚基编码mRNA之一的完整序列。
Biochimie. 1984 Jul-Aug;66(7-8):539-45. doi: 10.1016/0300-9084(84)90148-2.

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1
Transcription Terminator-Mediated Enhancement in Transgene Expression in Maize: Preponderance of the AUGAAU Motif Overlapping With Poly(A) Signals.转录终止子介导的玉米转基因表达增强：AUGAAU基序与多聚腺苷酸化信号重叠的优势
Front Plant Sci. 2020 Oct 14;11:570778. doi: 10.3389/fpls.2020.570778. eCollection 2020.
2
Synthetic versions of firefly luciferase and Renilla luciferase reporter genes that resist transgene silencing in sugarcane.萤火虫荧光素酶和海肾荧光素酶报告基因的合成版本，可抵抗甘蔗中的转基因沉默。
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Plant Mol Biol. 1987 Mar;10(2):127-37. doi: 10.1007/BF00016150.
5
A new member of the CAB gene family: structure, expression and chromosomal location of Cab-8, the tomato gene encoding the Type III chlorophyll a/b-binding polypeptide of photosystem I.一个 CAB 基因家族的新成员：结构、表达和染色体定位的 Cab-8，编码光系统 I Ⅲ型叶绿素 a/b 结合多肽的番茄基因。
Plant Mol Biol. 1989 Mar;12(3):257-70. doi: 10.1007/BF00043203.
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Nucleotide sequence and expression of the phytochrome gene in Pisum sativum: Differential regulation by light of multiple transcripts.豌豆光敏色素基因的核苷酸序列和表达：多种转录物的光差异调控。
Plant Mol Biol. 1988 Sep;11(5):697-710. doi: 10.1007/BF00017469.
7
The tomato polygalacturonase gene and ripening-specific expression in transgenic plants.番茄多聚半乳糖醛酸酶基因与转基因植物的成熟特异性表达。
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8
Drosophila P-element transcripts are incorrectly processed in tobacco.果蝇 P 元素转录本在烟草中被错误加工。
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9
Sequence comparisons of three wild-type Bronze-1 alleles from Zea mays.对来自玉米的三个野生型 Bronze-1 等位基因的序列比较。
Plant Mol Biol. 1988 Jul;11(4):473-81. doi: 10.1007/BF00039028.
10
Abundant embryonic mRNA in field bean (Vicia faba L.) codes for a new class of seed proteins: cDNA cloning and characterization of the primary translation product.富含胚胎 mRNA 的田菁（Vicia faba L.）编码一类新的种子蛋白：初级翻译产物的 cDNA 克隆和特性分析。
Plant Mol Biol. 1988 May;11(3):321-34. doi: 10.1007/BF00027389.

本文引用的文献

1
Linkage and homology analysis divides the eight genes for the small subunit of petunia ribulose 1,5-bisphosphate carboxylase into three gene families.连锁和同源分析将矮牵牛花核酮糖 1,5-二磷酸羧化酶小亚基的 8 个基因划分为三个基因家族。
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4964-8. doi: 10.1073/pnas.82.15.4964.
2
Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac).利用转座调控元件激活因子（Ac）通过简单且可推广的程序克隆玉米中的青铜基因座。
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3825-9. doi: 10.1073/pnas.81.12.3825.
3
Differential expression of the eight genes of the petunia ribulose bisphosphate carboxylase small subunit multi-gene family.矮牵牛核酮糖二磷酸羧化酶小亚基多基因家族 8 个基因的差异表达。
EMBO J. 1985 Dec 1;4(12):3055-61. doi: 10.1002/j.1460-2075.1985.tb04045.x.
4
Identification of sequences involved in the polyadenylation of higher plant nuclear transcripts using Agrobacterium T-DNA genes as models.以农杆菌T-DNA基因作为模型鉴定高等植物核转录本聚腺苷酸化所涉及的序列。
EMBO J. 1983;2(3):419-26. doi: 10.1002/j.1460-2075.1983.tb01439.x.
5
Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.根癌土壤杆菌Ti质粒编码的章鱼碱合酶基因的核苷酸序列和转录图谱。
J Mol Appl Genet. 1982;1(6):499-511.
6
Variation in the polyadenylylation site of bovine prolactin mRNA.牛催乳素mRNA聚腺苷酸化位点的变异
Proc Natl Acad Sci U S A. 1982 Jan;79(2):223-7. doi: 10.1073/pnas.79.2.223.
7
Requirement of a downstream sequence for generation of a poly(A) addition site.生成聚腺苷酸化位点对下游序列的要求。
Cell. 1984 Jul;37(3):993-9. doi: 10.1016/0092-8674(84)90433-1.
8
A number of different nuclear genes for the small subunit of RuBPCase are transcribed in petunia.矮牵牛中，编码1，5-二磷酸核酮糖羧化酶小亚基的多个不同核基因被转录。
Nucleic Acids Res. 1983 Jun 25;11(12):4177-83. doi: 10.1093/nar/11.12.4177.
9
Characterization of the expressed gene and several processed pseudogenes for the mouse ribosomal protein L30 gene family.小鼠核糖体蛋白L30基因家族的表达基因及几个加工假基因的特征分析。
Mol Cell Biol. 1984 Nov;4(11):2518-28. doi: 10.1128/mcb.4.11.2518-2528.1984.
10
Are U4 small nuclear ribonucleoproteins involved in polyadenylation?U4小核核糖核蛋白是否参与聚腺苷酸化过程？
Nature. 1984;309(5964):179-82. doi: 10.1038/309179a0.

几种植物基因的信使核糖核酸转录本在体内的多个位点进行多聚腺苷酸化。

mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo.

作者信息

Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J

出版信息

Nucleic Acids Res. 1986 Mar 11;14(5):2229-40. doi: 10.1093/nar/14.5.2229.

DOI:10.1093/nar/14.5.2229
PMID:3960719
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339654/
Abstract

We have analyzed the polyadenylation sites for the small subunit of ribulose bisphosphate carboxylase and chlorophyll a/b binding protein genes of Petunia (Mitchell) and the bronze gene of Zea mays. Sequence analysis of multiple cDNA clones revealed that polyadenylation of the transcripts occurred at either 2 or 3 sites for all three groups of genes. In the examples where 3 polyadenylation sites were detected, the middle site was the one predominantly used. Putative polyadenylation signals preceding the poly A tails diverged significantly from the animal consensus sequence AATAAA. In all the genes examined the first A residue in the poly A tail of the cDNA clones corresponded to an A residue in the homologous genomic sequence.

摘要

我们分析了矮牵牛（Mitchell）的1,5-二磷酸核酮糖羧化酶小亚基和叶绿素a/b结合蛋白基因以及玉米青铜基因的聚腺苷酸化位点。对多个cDNA克隆的序列分析表明，所有这三组基因的转录本的聚腺苷酸化发生在2个或3个位点。在检测到3个聚腺苷酸化位点的例子中，中间位点是主要使用的位点。聚腺苷酸尾巴之前的推定聚腺苷酸化信号与动物共有序列AATAAA有显著差异。在所有检测的基因中，cDNA克隆的聚腺苷酸尾巴中的第一个A残基与同源基因组序列中的一个A残基相对应。