Fanelli C, Castoria R, Fabbri A A, Passi S
Instituti Fisioterapici Ospedalieri, Ospedale S. Gallicano, Rome, Italy.
Nat Toxins. 1992;1(2):136-46. doi: 10.1002/nt.2620010213.
A GC-MS procedure was carried out for the simultaneous and unequivocal quantitation of both potato phytoalexin (rishitin and lubimin) accumulation and the rate of disappearance of polyunsaturated fatty acids (PUFA) and some of their esters tested as possible elicitors. Potato 5-lipoxygenase and lipolytic acyl hydrolase play a key role in hypersensitive response (HR) induction. As expected, arachidonic acid, its hydrolysable esters, and eicosapentaenoic acid elicited much higher HR than the other PUFA tested, although the latter were equally affected by potato 5-lipoxygenase. Hydroxyl radicals appear to be actively involved in the browning process. The polyaminoacid poly-L-lysine did not show any eliciting activity.
采用气相色谱-质谱联用（GC-MS）方法，对马铃薯植物抗毒素（日齐素和鲁比明）的积累以及作为可能诱导剂测试的多不饱和脂肪酸（PUFA）及其一些酯类的消失速率进行同时且明确的定量分析。马铃薯5-脂氧合酶和脂解酰基水解酶在超敏反应（HR）诱导中起关键作用。正如预期的那样，花生四烯酸、其可水解酯类和二十碳五烯酸引发的HR比其他测试的PUFA高得多，尽管后者同样受到马铃薯5-脂氧合酶的影响。羟基自由基似乎积极参与了褐变过程。聚氨基酸聚-L-赖氨酸未表现出任何诱导活性。
