随机扩增多态性DNA（RAPD）在分子生态学中的应用。

Applications of random amplified polymorphic DNA (RAPD) in molecular ecology.

作者信息

Hadrys H, Balick M, Schierwater B

机构信息

Yale University, Department of Biology, New Haven, CT 06511.

出版信息

Mol Ecol. 1992 May;1(1):55-63. doi: 10.1111/j.1365-294x.1992.tb00155.x.

DOI:10.1111/j.1365-294x.1992.tb00155.x

PMID:1344984

Abstract

Molecular genetic markers have been developed into powerful tools to analyse genetic relationships and genetic diversity. As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes. Main advantages of the RAPD technology include (i) suitability for work on anonymous genomes, (ii) applicability to problems where only limited quantities of DNA are available, (iii) efficiency and low expense.

摘要

分子遗传标记已发展成为分析遗传关系和遗传多样性的强大工具。作为对使用多态性DNA标记的现有各种技术的扩展，随机扩增多态性DNA（RAPD）技术可用于分子生态学，以确定分类身份、评估亲缘关系、分析混合基因组样本以及创建特异性探针。RAPD技术的主要优点包括：（i）适用于对未知基因组的研究；（ii）适用于DNA数量有限的问题；（iii）高效且成本低。