通过随机扩增多态性DNA（RAPD）分析评估日本梅花鹿种群内的遗传变异。
Assessment of genetic variations within populations of Sika deer in Japan by analysis of randomly amplified polymorphic DNA (RAPD).
作者信息
Tamate H B, Shibata K, Tsuchiya T, Ohtaishi N
机构信息
Department of Biotechnology, Senshu University of Ishinomaki, Japan.
出版信息
Zoolog Sci. 1995 Oct;12(5):669-73. doi: 10.2108/zsj.12.669.
Random amplified polymorphic DNA (RAPD) was exploited as a genetic marker to assess the level of genetic variation in populations of Sika deer, Cervus nippon, in Japan. DNA samples were collected from three local populations in Japan, namely, Kinkazan, Goyozan and Ashoro populations. Four arbitrary primers, when used individually, amplified an average of five RAPD fragments in the polymerase chain reaction (PCR). The number of polymorphic bands was scored to calculate band-sharing coefficients within populations. Average band-sharing coefficients revealed a higher degree of homogeneity in the Kinkazan population. Samples collected from larger populations, namely, Ashoro and Goyozan, revealed greater polymorphism than samples from the Kinkazan deer. Our data suggest that RAPD is useful as a marker for detecting genetic variations in populations of Sika deer with reduced levels of genetic diversity.
随机扩增多态性DNA（RAPD）被用作一种遗传标记，以评估日本梅花鹿（Cervus nippon）种群的遗传变异水平。从日本的三个当地种群，即金鵞山、御岳山和足寄种群采集了DNA样本。在聚合酶链反应（PCR）中，单独使用四条任意引物平均扩增出五个RAPD片段。对多态性条带的数量进行评分，以计算种群内的条带共享系数。平均条带共享系数显示金鵞山种群具有更高程度的同质性。从较大种群，即足寄和御岳山采集的样本显示出比金鵞山鹿样本更大的多态性。我们的数据表明，RAPD作为一种标记，可用于检测遗传多样性水平降低的梅花鹿种群中的遗传变异。
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