Zemelman R, Vejar C, Bello H, Domínguez M, González G
Departamento de Microbiología, Facultad de Ciencias Biológicas y de Recursos Naturales, Universidad de Concepción, Chile.
Rev Latinoam Microbiol. 1992 Oct-Dec;34(4):245-8.
The mechanisms of bactericidal activity of ciprofloxacin (mechanisms A and B) upon cells of a strain of Escherichia coli and one strain of Acinetobacter baumannii were investigated under different conditions. The killing of E. coli cells by ciprofloxacin was significantly reduced by chloramphenicol, but this antibiotic showed almost no activity upon killing of A. baumannii cells by this quinolone. Similar results were obtained when rifampicin was added to ciprofloxacin. Bactericidal activity of ciprofloxacin upon nondividing cells of E. coli was lower and that upon non-dividing cells of A. baumannii was not affected when compared with activity of ciprofloxacin upon dividing cells of both microorganisms. These results demonstrate that the antibacterial activity of ciprofloxacin upon A. baumannii is independent of protein and ARN synthesis, a fact which suggests that this quinolone exerts only bactericidal mechanism B upon A. baumannii. This finding might explain, at least in part, the lower susceptibility of this microorganism to ciprofloxacin.
在不同条件下，研究了环丙沙星对一株大肠杆菌和一株鲍曼不动杆菌细胞的杀菌活性机制（机制A和机制B）。氯霉素显著降低了环丙沙星对大肠杆菌细胞的杀伤作用，但这种抗生素对喹诺酮类药物杀伤鲍曼不动杆菌细胞几乎没有活性。当将利福平添加到环丙沙星中时，也获得了类似的结果。与环丙沙星对两种微生物分裂细胞的活性相比，环丙沙星对大肠杆菌非分裂细胞的杀菌活性较低，而对鲍曼不动杆菌非分裂细胞的杀菌活性没有影响。这些结果表明，环丙沙星对鲍曼不动杆菌的抗菌活性与蛋白质和RNA合成无关，这一事实表明这种喹诺酮类药物对鲍曼不动杆菌仅发挥杀菌机制B。这一发现可能至少部分解释了这种微生物对环丙沙星敏感性较低的原因。
