Figueroa J V, Alvarez J A, Buening G M, Cantó G J, Hernández R, Monroy M, Ramos J A, Vega C A
University of Missouri, Columbia 65211.
Rev Latinoam Microbiol. 1992 Oct-Dec;34(4):267-73.
To measure the antibody response to Babesia bigemina with an ELISA test, three groups of cattle were experimentally infected with two isolates of the parasite. It was possible to demonstrate specific antibody binding directed against the parasite as early as the 7 days postinfection (PI). The highest level of antibody was obtained around day 1 to 23 and remained detectable for 260 days. Challenge of the animals 260 days PI with a tick-induced B. bigemina infection depicted that homologous strain-challenged calves did not show an increase of IgG antibody levels, where as those challenged with the heterologous isolate did. In the latter groups the resulting level of antibodies was even higher than after the primary infection. The immunoblotting technique showed that the antibody response is probably directed against groups of B. bigemina components with a relative mobilities of 68-64 kDa, 62-54 kDa and 52-42 kDa, which appear to be major components of the protozoa. By observing the cross-reacting antigenicity among seven B. bigemina isolates, it was demonstrated that these components are not isolate-restricted.
为了通过酶联免疫吸附测定（ELISA）试验检测牛对双芽巴贝斯虫的抗体反应，将三组牛用该寄生虫的两个分离株进行实验性感染。早在感染后7天（PI）就有可能证明存在针对该寄生虫的特异性抗体结合。抗体水平在感染后第1天至23天左右达到最高，并在260天内均可检测到。在感染后260天，用蜱传播的双芽巴贝斯虫感染对动物进行攻毒，结果表明，用同源株攻毒的犊牛IgG抗体水平没有升高，而用异源分离株攻毒的犊牛则升高。在后一组中，产生的抗体水平甚至高于初次感染后。免疫印迹技术表明，抗体反应可能针对相对迁移率为68 - 64 kDa、62 - 54 kDa和52 - 42 kDa的双芽巴贝斯虫组分群，这些组分似乎是该原生动物的主要成分。通过观察七个双芽巴贝斯虫分离株之间的交叉反应抗原性，证明这些组分不受分离株限制。
