Knapp W, Ludwig H, Schernthaner G, Höfer R, Steffen C
Z Immunitatsforsch Exp Klin Immunol. 1976 Mar;151(1-2):61-8.
Previous investigations showed that quantitative immunofluorescence using antigens covalently bound to agarose particles represents a reproducible and sensitive assay for antibodies in experimental antisera. In this paper data are presented which show that also thyroglobulin antibodies in patients sera can be demonstrated using the defined antigen substrate spheres (DASS) system with thyroglobulin as antigen. Sera of 45 patients with various thyroid diseases were investigated for the presence of thyroglobulin antibodies using passive haemagglutination and the quantitative immunofluorescence technique. Comparable results were obtained with both techniques. Advantages and disadvantages of both methods are discussed.
先前的研究表明,使用与琼脂糖颗粒共价结合的抗原进行定量免疫荧光分析,是一种用于检测实验性抗血清中抗体的可重复且灵敏的方法。本文所呈现的数据表明,以甲状腺球蛋白作为抗原,使用特定抗原底物球(DASS)系统,也能够检测出患者血清中的甲状腺球蛋白抗体。本研究采用被动血凝试验和定量免疫荧光技术,对45例患有各种甲状腺疾病的患者血清进行检测,以确定其中是否存在甲状腺球蛋白抗体。两种技术获得了可比的结果。本文还讨论了这两种方法的优缺点。