Mariotti S, Pinchera A, Vitti P, Chiovato L, Marcocci C, Urbano C, Tosi M, Baschieri L
Clin Exp Immunol. 1978 Oct;34(1):118-25.
Parallel measurements of circulating anti-thyroid microsomal (anti-M) antibodies by radioassay and haemagglutination were performed on subjects with or without thyroid disorders. Three-quarters (75.4%) of control subjects had undetectable antibody levels (less than 10 u/ml) by radioassay and only 3.1% had concentrations of greater than or equal to 75 u/ml. Abnormally elevated levels (greater than or equal to 75 u/ml) were found in most of the patients with Hashimoto's thyroiditis (94.1%) or idiopathic myxoedema (86.7%), in the majority (75.0%) of those with Graves' disease and only in a minority of those with other thyroid disorders. The percentage of positive sera by haemagglutination was very similar in all groups to that of abnormal values observed in the radioassay. Direct comparison of parallel tests on a total of 631 sera revealed a highly significant correlation (r = 0.91, P less than 0.001) between the two methods, but elevated antibody titres by haemagglutination were found in some sera with negative radioassays. All these sera were from a single patient with thyroid carcinoma associated with Hashimoto's thyroiditis and had elevated levels of anti-thyroglobulin (anti-Tg) antibodies. Evidence that such discrepancies were due to anti-Tg antibodies reacting with microsomal-bound Tg was provided by the demonstration that the haemagglutination produced by these sera could be completely inhibited by the addition of Tg. A similar inhibition was observed with two rabbit antisera to human Tg, but not with sera from patients with thyroid autoimmune disorders containing high levels of anti-microsomal anti-bodies.
采用放射免疫分析法和血凝试验对患有或未患有甲状腺疾病的受试者进行循环抗甲状腺微粒体（抗-M）抗体的平行检测。通过放射免疫分析法，四分之三（75.4%）的对照受试者抗体水平检测不到（低于10 u/ml），只有3.1%的受试者抗体浓度大于或等于75 u/ml。在大多数桥本甲状腺炎患者（94.1%）或特发性黏液性水肿患者（86.7%）中，以及大多数格雷夫斯病患者（75.0%）中发现抗体水平异常升高（大于或等于75 u/ml），而在其他甲状腺疾病患者中只有少数出现这种情况。血凝试验阳性血清的百分比在所有组中与放射免疫分析法中观察到的异常值百分比非常相似。对总共631份血清进行的平行试验直接比较显示，两种方法之间存在高度显著的相关性（r = 0.91，P < 0.001），但在一些放射免疫分析为阴性的血清中发现血凝试验抗体滴度升高。所有这些血清均来自一名患有与桥本甲状腺炎相关的甲状腺癌的患者，其抗甲状腺球蛋白（抗-Tg）抗体水平升高。通过证明这些血清产生的血凝反应可被添加的Tg完全抑制，提供了此类差异是由于抗-Tg抗体与微粒体结合的Tg反应所致的证据。用两种抗人Tg兔抗血清观察到类似的抑制作用，但含有高水平抗微粒体抗体的甲状腺自身免疫性疾病患者的血清未观察到这种抑制作用。
