Vallejo G A, Marinkelle C J
Departamento de Biología, Universidad de Tolima, Ibagué, Colombia.
Rev Latinoam Microbiol. 1992 Apr-Jun;34(2):91-4.
Using three different methods, the activity of neuraminidase was studied in the promesenteron, postmesenteron, rectal ampulla, haemolymph and salivary glands in 600 Rhodnius prolixus experimentally infected with Trypanosoma rangeli stock San Agustín. The haemagglutination method with peanut lectin, and the fluorescence test with peanut lectin conjugated with fluorescein isothiocyanate, and the fluorescence emitted by 4-methylumbelliferone showed in all cases the presence of neuraminidase in the supernatant culture of T. rangeli in Tobie's medium between 8 to 15 days growth. None of the three methods was able to detect the presence of neuraminidase in R. prolixus infected with T. Rangeli, thus suggesting that this enzyme is not produced in vivo, and consecutively is not implicated in the pathogenicity that this trypanosome has to its vector.
采用三种不同方法，对600只经实验感染克氏锥虫圣奥古斯丁株的红带锥蝽的前肠、中肠后段、直肠壶腹、血淋巴和唾液腺中的神经氨酸酶活性进行了研究。用花生凝集素的血凝方法、用异硫氰酸荧光素偶联的花生凝集素的荧光试验以及4-甲基伞形酮发出的荧光，在所有情况下均显示在托比氏培养基中生长8至15天的克氏锥虫上清培养物中存在神经氨酸酶。这三种方法均未能检测到感染克氏锥虫的红带锥蝽中神经氨酸酶的存在，因此表明该酶并非在体内产生，进而表明其与该锥虫对其媒介的致病性无关。
