Bièche I, Champème M H, Matifas F, Hacène K, Callahan R, Lidereau R
Oncovirology Laboratory, Centre René Huguenin, St-Cloud, France.
Lancet. 1992 Jan 18;339(8786):139-43. doi: 10.1016/0140-6736(92)90208-k.
Genetic alterations are believed to be important in the origin and dissemination of breast cancer. Cytogenetic rearrangements on chromosome 7 are common in breast tumours. We used the c-met proto-oncogene probe, which detects sequences on chromosome 7q31, to analyse tumour and blood leucocyte DNA samples from 245 patients with primary breast cancers. The pmetH polymorphic probe detected a high frequency of allele loss (40.5%) among the 121 informative (heterozygous) patients. This genetic alteration was not significantly associated with standard prognostic features including tumour size, histopathological grade, and lymph-node or steroid receptor status. However, patients with loss of heterozygosity on chromosome 7q31 in primary tumour DNA had significantly shorter metastasis-free survival (p = 0.00022) and overall survival (p = 0.0036) after surgery than patients without this alteration. These findings indicate that this region of chromosome 7 might be the site of a breast tumour or metastasis suppressor gene.
基因改变被认为在乳腺癌的起源和扩散中起着重要作用。7号染色体上的细胞遗传学重排在乳腺肿瘤中很常见。我们使用检测7q31染色体序列的c-met原癌基因探针，分析了245例原发性乳腺癌患者的肿瘤和血液白细胞DNA样本。pmetH多态性探针在121例信息丰富（杂合子）的患者中检测到高频等位基因缺失（40.5%）。这种基因改变与包括肿瘤大小、组织病理学分级以及淋巴结或类固醇受体状态等标准预后特征没有显著相关性。然而，原发性肿瘤DNA中7q31染色体杂合性缺失的患者术后无转移生存期（p = 0.00022）和总生存期（p = 0.0036）明显短于无此改变的患者。这些发现表明，7号染色体的这个区域可能是乳腺肿瘤或转移抑制基因的位点。
