Bieche I, Champeme M H, Merlo G, Larsen C J, Callahan R, Lidereau R
Centre René Huguenin, St. Cloud, France.
Hum Genet. 1990 Jun;85(1):101-5. doi: 10.1007/BF00276332.
Recent studies suggest that loss of heterozygosity may play an important role in various human neoplasia. Cytogenetic abnormalities detected in primary breast tumors led us to examine breast tumor DNAs for deletions. In the present study, we demonstrate, using restriction fragment length polymorphism (RFLP) analysis at the L-myc proto-oncogene (chromosome 1p32), a frequent loss of heterozygosity in primary breast tumor DNAs (55 out of 152 informative tumor DNAs). Most of these deletions appear to be limited to chromosome 1p. No correlation was observed between this genetic alteration and several parameters of each patient's history or characteristics of the tumor. However, a significantly (P = 0.011) shorter survival period after relapse was observed for patients with loss of heterozygosity at L-myc in primary tumor DNAs compared with patients with tumor DNAs lacking this alteration.
近期研究表明，杂合性缺失可能在各种人类肿瘤形成过程中发挥重要作用。在原发性乳腺肿瘤中检测到的细胞遗传学异常促使我们检测乳腺肿瘤DNA是否存在缺失。在本研究中，我们利用位于L - myc原癌基因（染色体1p32）处的限制性片段长度多态性（RFLP）分析表明，原发性乳腺肿瘤DNA中频繁出现杂合性缺失（152份信息丰富的肿瘤DNA中有55份）。这些缺失大多似乎局限于染色体1p。未观察到这种基因改变与每位患者病史的几个参数或肿瘤特征之间存在相关性。然而，与肿瘤DNA无此改变的患者相比，原发性肿瘤DNA中L - myc杂合性缺失的患者复发后的生存期显著缩短（P = 0.011）。
