VANE J R
Br J Pharmacol Chemother. 1957 Sep;12(3):344-9. doi: 10.1111/j.1476-5381.1957.tb00146.x.
The movements of a strip of fundus from a rat stomach, suspended in a 5 ml. bath at 37 degrees , are recorded by a spring-loaded lever. The muscle is stretched for 15 to 30 sec. between contractions. 1 ng. 5-Hydroxytryptamine (5-HT), 10 ng. acetylcholine or 2 to 10 mug. of histamine all produce contractions, giving tracings about 4 cm. high. In the presence of hyoscine (10(-7)) low concentrations of 5-HT can be assayed directly from mixtures of the three substances. Sympathomimetic amines interfere with the assay, but this can be overcome. The preparation is robust, potentially suitable for use with an automatic assay apparatus and 10 to 100 times more sensitive than the rat uterus.
将大鼠胃的一片胃底组织条悬于5毫升、37摄氏度的浴槽中,其运动由一个弹簧加载杠杆记录。肌肉在两次收缩之间伸展15至30秒。1纳克5-羟色胺(5-HT)、10纳克乙酰胆碱或2至10微克组胺均可引起收缩,记录曲线约4厘米高。在存在东莨菪碱(10⁻⁷)的情况下,低浓度的5-HT可直接从这三种物质的混合物中进行测定。拟交感胺会干扰测定,但这一问题可以克服。该制备方法稳定,有可能适用于自动测定仪器,且比大鼠子宫敏感10至100倍。