CLEUGH J, GADDUM J H, HOLTON P, LEACH E
Br J Pharmacol Chemother. 1961 Aug;17(1):144-58. doi: 10.1111/j.1476-5381.1961.tb01115.x.
The optimum conditions for the assay of substance P on fowl rectal caecum have been studied. Effective concentrations vary from 0.01 to 0.5 u./ml.; it is remarkably insensitive to other polypeptides, such as bradykinin. The test can be made more specific by using a bath fluid containing antagonists for known interfering substances. A suitable antagonist for acetylcholine is atropine or hyoscine, for 5-hydroxytryptamine, methysergide and for catecholamines, ephedrine. The effects of histamine and adenosine compounds can be abolished by specific tachyphylaxis, in which the bath fluid contains an excess of the active substance itself. The assay is reasonably accurate. Woolf's index of precision (L) was estimated as 15.5 with a range (9) of 9.2 to 29.4. Simplified methods of calculating L, and the fiducial range, are described.
对鸡直肠盲肠中P物质测定的最佳条件进行了研究。有效浓度范围为0.01至0.5单位/毫升；它对其他多肽（如缓激肽）非常不敏感。通过使用含有已知干扰物质拮抗剂的浴液，可使测试更具特异性。乙酰胆碱的合适拮抗剂是阿托品或东莨菪碱，5-羟色胺的拮抗剂是甲基麦角新碱，儿茶酚胺的拮抗剂是麻黄碱。组胺和腺苷化合物的作用可通过特异性快速脱敏消除，其中浴液含有过量的活性物质本身。该测定相当准确。伍尔夫精密度指数（L）估计为15.5，范围（9）为9.2至29.4。描述了计算L和置信区间的简化方法。
