Fisher R A, Newlands E S, Jeffreys A J, Boxer G M, Begent R H, Rustin G J, Bagshawe K D
Department of Genetics, University of Leicester, England.
Cancer. 1992 Feb 1;69(3):839-45. doi: 10.1002/1097-0142(19920201)69:3<839::aid-cncr2820690336>3.0.co;2-e.
In three patients in whom a diagnosis of gestational trophoblastic tumor was possible on the basis of pathology and elevated levels of serum human chorionic gonadotrophin, locus-specific minisatellite probes were used to identify restriction fragment length polymorphisms (RFLP) in DNA from the tumor, the patient, and her partner. On the basis of results from these studies, one tumor, originally diagnosed as a germ cell tumor, was reclassified as a gestational choriocarcinoma, whereas a second tumor, diagnosed as gestational choriocarcinoma, was shown to be of nongestational origin. In the third case, a diagnosis of gestational trophoblastic tumor was confirmed, but in this case the androgenetic origin of the tumor indicated that it was derived, not from the antecedent term pregnancy, but from a previous pregnancy with hydatidiform mole. This study clearly demonstrates the value of DNA analysis in the classification of tumors with trophoblastic differentiation.
在三名根据病理学检查及血清人绒毛膜促性腺激素水平升高可能诊断为妊娠滋养细胞肿瘤的患者中，使用位点特异性小卫星探针来鉴定肿瘤、患者及其配偶DNA中的限制性片段长度多态性（RFLP）。基于这些研究结果，一例最初诊断为生殖细胞肿瘤的肿瘤被重新分类为妊娠绒毛膜癌，而另一例诊断为妊娠绒毛膜癌的肿瘤被证明是非妊娠起源。在第三例中，妊娠滋养细胞肿瘤的诊断得到证实，但在该病例中肿瘤的孤雄起源表明它并非来自前次足月妊娠，而是来自先前的葡萄胎妊娠。这项研究清楚地证明了DNA分析在滋养细胞分化肿瘤分类中的价值。
