Suzuki T, Goto S, Nawa A, Kurauchi O, Saito M, Tomoda Y
Department of Obstetrics and Gynecology, Nagoya University, School of Medicine, Japan.
Obstet Gynecol. 1993 Oct;82(4 Pt 1):629-34.
In three cases of choriocarcinoma, genetic loci including a variable number of tandem repeat regions were amplified by the polymerase chain reaction method on DNA from three established cell lines and from lymphocytes of patients and their husbands to identify the responsible pregnancy. Case 1, from whom NaUCC-3 was derived, had only one full-term fetal death. Case 2, from whom NaUCC-4 was derived, had one normal delivery followed by one complete molar delivery and one normal delivery. Case 3, from whom NaUCC-2 was derived, had one normal delivery followed by one complete molar delivery. In case 1, NaUCC-3 was found to be of parental origin and derived from the pregnancy with full-term fetal death. In cases 2 and 3, NaUCC-4 and NaUCC-2 were of probable androgenetic origin and were derived from the pregnancy with complete hydatidiform mole. We also conducted the restriction fragment length polymorphism method using case 1 samples, and it confirmed the results based on the polymerase chain reaction method product patterns. All nine cases of hydatidiform mole and three cases of invasive mole were of androgenetic origin. The polymerase chain reaction method thus makes it possible to identify easily the pregnancy responsible for choriocarcinoma using only a few specimens without isotopes.
在3例绒毛膜癌病例中，采用聚合酶链反应方法，对来自3个已建立的细胞系以及患者及其丈夫淋巴细胞的DNA中的包括可变数量串联重复区域在内的基因位点进行扩增，以确定相关妊娠情况。病例1衍生出NaUCC - 3，其仅有一次足月胎儿死亡。病例2衍生出NaUCC - 4，其有一次正常分娩，随后是一次完全性葡萄胎分娩和一次正常分娩。病例3衍生出NaUCC - 2，其有一次正常分娩，随后是一次完全性葡萄胎分娩。在病例1中，发现NaUCC - 3来自父母，且源自伴有足月胎儿死亡的那次妊娠。在病例2和3中，NaUCC - 4和NaUCC - 2可能源自孤雄生殖，且源自伴有完全性葡萄胎的那次妊娠。我们还对病例1的样本进行了限制性片段长度多态性分析，其根据聚合酶链反应方法的产物模式证实了结果。所有9例葡萄胎和3例侵袭性葡萄胎均源自孤雄生殖。因此，聚合酶链反应方法仅使用少量样本且无需同位素，就能够轻松鉴定出与绒毛膜癌相关的妊娠情况。
