Wang M, Mitchell C J, Hu J S, Gonsalves D, Calisher C H
Plant Pathology Department, Cornell University, New York State Agricultural Experiment Station, Geneva.
Intervirology. 1992;33(1):32-40. doi: 10.1159/000150228.
Tomato spotted wilt virus (TSWV) has been reported to be morphologically, molecularly and structurally similar to viruses in the family Bunyaviridae. By various types of enzyme-linked immunosorbent assays (ELISA) and Western blot hybridizations, we tested TSWV with antibodies to 12 viruses in the Phlebovirus genus of this family. Serological relatedness was not found between TSWV and phleboviruses. However, one preparation of antibody to Arumowot virus reacted with a 53-kD protein from healthy plant extracts. Six-day-old adult Toxorhynchites amboinensis mosquitoes were inoculated with purified TSWV. Infectious virus was not detected in any of the injected insects during the 5-week test period. However, TSWV antigens were detected in these mosquitoes by ELISA at the original injected level for at least a week after injection. TSWV antigen concentration began to decrease thereafter, but remained at detectable levels for as long as 5 weeks after injection. However, there was no evidence that TSWV replicated in mosquitoes.
据报道，番茄斑萎病毒（TSWV）在形态、分子和结构上与布尼亚病毒科的病毒相似。通过各种类型的酶联免疫吸附测定（ELISA）和蛋白质印迹杂交，我们用针对该科白蛉病毒属中12种病毒的抗体检测了TSWV。未发现TSWV与白蛉病毒之间存在血清学相关性。然而，一种抗阿鲁莫沃特病毒的抗体制剂与来自健康植物提取物的一种53-kD蛋白发生了反应。用纯化的TSWV接种6日龄的成年amboinensis巨蚊。在为期5周的测试期内，在任何一只注射的昆虫中均未检测到感染性病毒。然而，通过ELISA在这些蚊子中检测到TSWV抗原，在注射后至少一周内保持在最初注射的水平。此后，TSWV抗原浓度开始下降，但在注射后长达5周内仍保持在可检测水平。然而，没有证据表明TSWV在蚊子中复制。
