Augood S J, Emson P C
MRC Group, Department of Neuroendocrinology, AFRC Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, England.
Synapse. 1992 Jan;10(1):16-24. doi: 10.1002/syn.890100104.
Endogenous cytochrome oxidase activity was investigated in the adult rat striatum at the light microscope level to see if it was distributed in accordance with the established striatal patch/matrix compartmentalisation. Striatal sections stained to visualise cytochrome oxidase activity were compared with serial sections stained to visualise tyrosine hydroxylase and calbindinD28k-like immunoreactivity, established markers of the matrix compartment. The distribution of endogenous cytochrome oxidase activity was found to coincide with the immunocytochemical staining pattern seen for tyrosine hydroxylase and calbindinD28k whereby areas of intense tyrosine hydroxylase and calbindinD28k-like immunoreactivity (termed the matrix) corresponded to areas of intense cytochrome oxidase activity. Conversely, areas of less intense tyrosine hydroxylase and calbindinD28k-like immunoreactivity (termed patches) corresponded to areas of low cytochrome oxidase activity. In addition, the distribution of two other oxidative enzymes involved in the regulation of mitochondrial respiration, succinic dehydrogenase and NADH-diaphorase, was examined in the striatum and substantia nigra by using histochemical techniques. Both NADH-diaphorase and succinic dehydrogenase histochemistry showed an uneven pattern of neuropil staining in the striatum. In the substantia nigra a few intensely stained cell bodies were seen in the dorsal-lateral tip of the pars reticulata with both histochemical techniques. By using an anti-cytochrome oxidase antibody an abundance of immunoreactive cell bodies and processes were seen in the substantia nigra, particularly in the dorso-medial rim and dorsal tip of the pars reticulata. The substantia nigra pars lateralis contained many intensely stained cytochrome oxidase-like immunoreactive cell bodies and processes.(ABSTRACT TRUNCATED AT 250 WORDS)
在光学显微镜水平上研究成年大鼠纹状体中的内源性细胞色素氧化酶活性，以观察其分布是否符合已确立的纹状体斑块/基质分区。将用于观察细胞色素氧化酶活性的纹状体切片与用于观察酪氨酸羟化酶和钙结合蛋白D28k样免疫反应性（基质分区的既定标志物）的连续切片进行比较。发现内源性细胞色素氧化酶活性的分布与酪氨酸羟化酶和钙结合蛋白D28k的免疫细胞化学染色模式一致，即酪氨酸羟化酶和钙结合蛋白D28k样免疫反应性强烈的区域（称为基质）对应于细胞色素氧化酶活性强烈的区域。相反，酪氨酸羟化酶和钙结合蛋白D28k样免疫反应性较弱的区域（称为斑块）对应于细胞色素氧化酶活性较低的区域。此外，使用组织化学技术在纹状体和黑质中检测了另外两种参与线粒体呼吸调节的氧化酶——琥珀酸脱氢酶和NADH-黄递酶的分布。NADH-黄递酶和琥珀酸脱氢酶组织化学均显示纹状体中神经毡染色不均匀。在黑质中，两种组织化学技术均在网状部背外侧尖端观察到一些染色强烈的细胞体。通过使用抗细胞色素氧化酶抗体，在黑质中观察到大量免疫反应性细胞体和突起，特别是在网状部的背内侧边缘和背尖端。黑质外侧部含有许多染色强烈的细胞色素氧化酶样免疫反应性细胞体和突起。（摘要截短于250字）
