Pareyre C, Farjaudon N, Deysson G
C R Seances Soc Biol Fil. 1976;170(1):14-9.
A population of meristematic root cells of Allium sativum L. is previously synchronized thanks to Hydroxyurea. When it reaches mitosis, this population undergoes the action of different antimetabolic drugs which slightly check the normal operating of the mitotic spindle without preventing chromosomic separation. In such conditions we can obtain from 30 to 50 slightly altered anaphasic cells out of 1000 cells. This is enough to allow us to contemplate the electronic microscope study of these abnormalities.
由于羟基脲的作用,大蒜的一群分生组织根细胞先前已实现同步化。当它进入有丝分裂时,这群细胞会受到不同抗代谢药物的作用,这些药物会轻微抑制有丝分裂纺锤体的正常运作,但不会阻止染色体分离。在这种条件下,我们从1000个细胞中可以获得30到50个有轻微改变的后期细胞。这足以让我们考虑对这些异常情况进行电子显微镜研究。