向日葵一种新型细胞质雄性不育基因型的分子分析

Molecular analysis of a new cytoplasmic male sterile genotype in sunflower.

作者信息

Spassova M, Christov M, Bohorova N, Petrov P, Dudov K, Atanassov A, Nijkamp H J, Hille J

机构信息

Institute of Genetic Engineering, Kostinbrod, Bulgaria.

出版信息

FEBS Lett. 1992 Feb 3;297(1-2):159-63. doi: 10.1016/0014-5793(92)80350-p.

DOI:10.1016/0014-5793(92)80350-p

PMID:1348035

Abstract

Mitochondrial DNA from 1 fertile and 6 cytoplasmic male sterile (CMS) sunflower genotypes was studied. The CMS genotypes had been obtained either by specific crosses between different Helianthus species or by mutagenesis. CMS-associated restriction fragment length polymorphisms (RFLPs) were found in the vicinity of the atpA locus, generated by various restriction enzymes. The organization of the mitochondrial genes 26S rRNA, 18S + 5S rRNA and coxII was investigated by Southern blot analysis. These genes have similar structures in fertile and all studied sterile sources. Using the atpA probe, 5 from the 6 investigated CMS genotypes showed identical hybridization patterns to the Petiolaris CMS line, which is used in all commercial sunflower hybrids. Only 1 cytoplasm derived from an open pollination of Helianthus annuus ssp. texanus, known as ANT1, contained a unique mitochondrial DNA fragment, which is distinguishable from the fertile and sterile Petiolaris genotypes and from all investigated CMS genotypes. Male fertility restoration and male sterility maintenance of the ANT1 line are different from the Petiolaris CMS system, which is a confirmation that a novel CMS genotype in sunflower has been identified.

摘要

对1个可育向日葵基因型和6个细胞质雄性不育（CMS）向日葵基因型的线粒体DNA进行了研究。这些CMS基因型是通过不同向日葵物种之间的特定杂交或诱变获得的。在atpA基因座附近发现了与CMS相关的限制性片段长度多态性（RFLP），这些多态性是由各种限制性酶产生的。通过Southern印迹分析研究了线粒体基因26S rRNA、18S + 5S rRNA和coxII的组织情况。在可育和所有研究的不育来源中，这些基因具有相似的结构。使用atpA探针，6个被研究的CMS基因型中有5个与所有商业向日葵杂交种中使用的叶柄CMS系显示出相同的杂交模式。只有1个源自向日葵亚种德克萨斯向日葵开放授粉的细胞质，称为ANT1，包含一个独特的线粒体DNA片段，该片段与可育和不育的叶柄基因型以及所有研究的CMS基因型都不同。ANT1系的雄性育性恢复和雄性不育维持与叶柄CMS系统不同，这证实了已鉴定出一种新的向日葵CMS基因型。