Köhler R H, Horn R, Lössl A, Zetsche K
Institut für Pflanzenphysiologie, Justus-Liebig-Universität, Giessen, FRG.
Mol Gen Genet. 1991 Jul;227(3):369-76. doi: 10.1007/BF00273925.
The organization and expression of the mitochondrial (mt) genome of fertile, male-sterile and restored lines of Helianthus annuus and of H. petiolaris were compared to identify alterations which might lead to cytoplasmic male sterility (CMS). The mtDNAs of fertile and male-sterile lines differ by an 11 kb inversion and a 5 kb insertion. The rearrangements seem to be the result of recombination events within an inverted repeat of 261 bp. Detectable alterations in the transcript pattern of the rearranged mtDNA regions are restricted to the atpA locus. The male-sterile line CMSBaso shows three additional transcripts of the atpA locus of about 2500, 1200 and 250 nucleotides which are not detectable in Baso. However, the coding sequences of the atpA gene are entirely identical in the fertile line Baso and the male-sterile line CMSBaso. But a new open reading frame (orfH522) of 522 nucleotides is co-transcribed with the atpA gene as an additional larger transcript of about 2500 nucleotides in CMSBaso. orfH522 is also included in a second additional transcript of about 1200 nucleotides. The predicted translation product of orfH522 might play a role in CMS in sunflower.
对向日葵和叶柄向日葵可育系、雄性不育系及恢复系的线粒体（mt）基因组的组织和表达进行了比较，以确定可能导致细胞质雄性不育（CMS）的改变。可育系和雄性不育系的线粒体DNA（mtDNA）存在11 kb的倒位和5 kb的插入差异。这些重排似乎是261 bp反向重复序列内重组事件的结果。重排的mtDNA区域转录模式的可检测改变仅限于atpA基因座。雄性不育系CMSBaso的atpA基因座显示出另外三种转录本，大小约为2500、1200和250个核苷酸，而在Baso中无法检测到。然而，可育系Baso和雄性不育系CMSBaso中atpA基因的编码序列完全相同。但是，在CMSBaso中，一个522个核苷酸的新开放阅读框（orfH522）与atpA基因共同转录，形成一个约2500个核苷酸的额外更大转录本。orfH522也包含在另一个约1200个核苷酸的转录本中。orfH522的预测翻译产物可能在向日葵细胞质雄性不育中起作用。
