用冻干法保存的粗糙脉孢菌突变体进行维生素测定的可重复性。

Reproducibility of vitamin assays with mutants of Neurospora crassa maintained by freeze-drying.

作者信息

Jong S C, Davis E E

出版信息

Can J Microbiol. 1976 Jul;22(7):1062-4. doi: 10.1139/m76-156.

Abstract

Three mutant strains (ATCC 9277, ATCC 9278, ATCC 9683) of Neurospora crassa requiring choline, inositol, and p-aminobenzoic acid respectively for normal growth were deposited at the ATCC by G.W. Beadle 30 years ago and were preserved at various time intervals by freeze-drying. Each preservation batch yielded cultures that, when used for biological assays, exhibited the same biochemical properties as they originally possessed. The freeze-drying technique is shown to be applicable to preserving biological properties of sporulating fungi for bioassay over prolonged periods of time.

摘要

三种粗糙脉孢菌突变菌株（ATCC 9277、ATCC 9278、ATCC 9683），分别需要胆碱、肌醇和对氨基苯甲酸才能正常生长，30年前由G.W.比德尔保藏于美国典型培养物保藏中心（ATCC），并通过冷冻干燥在不同时间间隔进行保存。每个保存批次所产生的培养物，用于生物测定时，都表现出与它们最初所具有的相同生化特性。结果表明，冷冻干燥技术适用于长期保存产孢真菌的生物学特性以用于生物测定。