利用DNA探针分析日本瑟氏泰勒虫虫株的基因组

Genomic analysis of Theileria sergenti stocks in Japan with DNA probes.

作者信息

Matsuba T, Kawakami Y, Iwai H, Onuma M

机构信息

Department of Veterinary Microbiology, Rakuno Gakuen University, Ebetsu, Japan.

出版信息

Vet Parasitol. 1992 Feb;41(1-2):35-43. doi: 10.1016/0304-4017(92)90006-u.

DOI:10.1016/0304-4017(92)90006-u

PMID:1348593

Abstract

Restriction fragment length polymorphisms of Theileria sergenti DNA from 18 different infections of cattle in 14 locations in Japan were analyzed by Southern blotting using T. sergenti genomic DNA fragments as probes. Probe pTs 2 hybridized with four fragments in BamHI digested piroplasm DNA, at 8.0, 7.3, 6.0 and 3.4 kb. Probe pTs 11-D1 hybridized with multiple fragments. With each probe, polymorphisms were observed among stocks from different locations. However, there was no correlation between the patterns of hybridization bands and the locations where parasites were collected. Analysis of the hybridization patterns of stocks obtained from individual cattle in the same grazing areas showed an almost identical pattern.

摘要

利用瑟氏泰勒虫基因组DNA片段作为探针，通过Southern印迹法分析了来自日本14个地点18种不同牛感染的瑟氏泰勒虫DNA的限制性片段长度多态性。探针pTs 2与经BamHI消化的梨形虫DNA中的4个片段杂交，片段大小分别为8.0、7.3、6.0和3.4 kb。探针pTs 11-D1与多个片段杂交。使用每种探针时，在不同地点的虫株之间均观察到多态性。然而，杂交带型与采集寄生虫的地点之间没有相关性。对来自同一放牧区个体牛的虫株杂交模式分析显示，其模式几乎相同。