感染期间瑟氏泰勒虫群体杂交模式的变化。

Changes in the hybridization patterns of populations of Theileria sergenti during infection.

作者信息

Matsuba T, Sugimoto C, Onoe S, Kawakami Y, Iwai H, Onuma M

机构信息

Department of Veterinary Microbiology, Rakuno Gakuen University, Ebetsu, Japan.

出版信息

Vet Parasitol. 1993 May;47(3-4):215-23. doi: 10.1016/0304-4017(93)90023-g.

DOI:10.1016/0304-4017(93)90023-g

PMID:8101404

Abstract

Restriction fragment length polymorphisms (RFLPs) of Theileria sergenti DNA were analysed using probes of a genomic DNA fragment (pTs 2) and a cDNA corresponding to this genomic probe (C-Ts 2). Each of the probes detected RFLPs in DNA from different stocks of Theileria sergenti. Additionally, using these probes, alterations in hybridization patterns were observed in samples of the parasites harvested at different times after individual calves had been infected with Theileria sergenti. This result suggests that the Theileria sergenti stocks used were mixed parasite populations.

摘要

使用基因组DNA片段（pTs 2）的探针和与该基因组探针对应的cDNA（C-Ts 2）分析了瑟氏泰勒虫DNA的限制性片段长度多态性（RFLP）。每个探针都检测到了来自不同瑟氏泰勒虫虫株的DNA中的RFLP。此外，使用这些探针，在个体犊牛感染瑟氏泰勒虫后不同时间采集的寄生虫样本中观察到杂交模式的变化。这一结果表明所使用的瑟氏泰勒虫虫株是混合的寄生虫群体。