Ducharme J, Varin F, Bevan D R, Donati F, Théorêt Y
Faculté de Pharmacie, Université de Montréal, Quebec, Canada.
J Chromatogr. 1992 Jan 3;573(1):79-86. doi: 10.1016/0378-4347(92)80477-8.
A high-performance liquid chromatographic assay coupled with electrochemical detection has been developed for the determination of vecuronium and its three putative deacetylated metabolites in human plasma. A novel solid-phase extraction procedure allowed good recovery of both vecuronium and its metabolites, together with ease and speed of execution. This method was sensitive, reproducible and accurate over the therapeutic range of concentrations of vecuronium and its metabolites, and was applied successfully to a study of the pharmacokinetics of vecuronium in anaesthetized patients.
已开发出一种结合电化学检测的高效液相色谱分析法，用于测定人血浆中维库溴铵及其三种假定的脱乙酰代谢物。一种新颖的固相萃取方法能够很好地回收维库溴铵及其代谢物，且操作简便、快速。该方法在维库溴铵及其代谢物的治疗浓度范围内灵敏、可重复且准确，并成功应用于麻醉患者维库溴铵的药代动力学研究。
