Paanakker J E, Thio J M, Van den Wildenberg H M, Kaspersen F M
Organon International B.V., Scientific Development Group, Oss, The Netherlands.
J Chromatogr. 1987 Oct 30;421(2):327-35. doi: 10.1016/0378-4347(87)80412-7.
An assay has been developed and validated for the routine monitoring of vecuronium in plasma. It consists of solid-phase extraction using C18 disposables as sample pre-treatment, high-performance liquid chromatography and post-column ion-pair extraction with fluorimetric detection. The fluorescent anion 9,10-dimethoxyanthracene-2-sulphonate (DAS) is used as the counter ion. The detection limit is ca. 5 ng/ml in plasma with a signal-to-noise ratio of 10. The assay is also applicable for monitoring vecuronium and its potential metabolites in other biological media, e.g., urine, bile and tissue (liver, kidney) homogenates.
已开发并验证了一种用于血浆中维库溴铵常规监测的分析方法。该方法包括使用C18一次性萃取柱进行固相萃取作为样品预处理、高效液相色谱法以及柱后离子对萃取结合荧光检测。荧光阴离子9,10-二甲氧基蒽-2-磺酸盐（DAS）用作反离子。血浆中的检测限约为5 ng/ml，信噪比为10。该分析方法也适用于监测其他生物介质（如尿液、胆汁以及组织（肝脏、肾脏）匀浆）中的维库溴铵及其潜在代谢物。
