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辅酶A是大鼠附睾脂肪垫中乙酰辅酶A羧化酶的有效抑制剂。

Coenzyme A is a potent inhibitor of acetyl-CoA carboxylase from rat epididymal fat-pads.

作者信息

Moule S K, Edgell N J, Borthwick A C, Denton R M

机构信息

Department of Biochemistry, School of Medical Sciences, Bristol, U.K.

出版信息

Biochem J. 1992 Apr 1;283 ( Pt 1)(Pt 1):35-8. doi: 10.1042/bj2830035.

DOI:10.1042/bj2830035
PMID:1348928
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1130988/
Abstract

Rat epididymal fat-pad extracts have previously been shown to contain an insulin-stimulated acetyl-CoA carboxylase kinase, which is co-eluted from Mono Q ion-exchange chromatography with a potent inhibitor of acetyl-CoA carboxylase [Borthwick, Edgell & Denton (1990) Biochem. J. 270, 795-801]. A variety of tests, including reactivity with thiol reagents, identify this inhibitor as CoA. Inhibition requires the presence of MgATP, but is independent of any phosphorylation of the enzyme. The effect is complete in about 5 min and is associated with depolymerization of acetyl-CoA carboxylase. Half-maximal inhibition is observed at about 40 nM-CoA. The inhibitory effects of CoA can be partially reversed by incubation with citrate and more fully overcome by treatment of the enzyme with the insulin-stimulated acetyl-CoA carboxylase kinase.

摘要

先前已表明，大鼠附睾脂肪垫提取物含有一种胰岛素刺激的乙酰辅酶A羧化酶激酶，该激酶与乙酰辅酶A羧化酶的一种强效抑制剂在Mono Q离子交换色谱中共同洗脱[博思威克、埃杰尔和丹顿（1990年）《生物化学杂志》270卷，795 - 801页]。包括与硫醇试剂反应性在内的各种测试表明，这种抑制剂是辅酶A。抑制作用需要MgATP的存在，但与该酶的任何磷酸化无关。这种作用在约5分钟内完成，并且与乙酰辅酶A羧化酶的解聚有关。在约40 nM - 辅酶A时观察到半数最大抑制。通过与柠檬酸盐一起孵育，辅酶A的抑制作用可部分逆转，而用胰岛素刺激的乙酰辅酶A羧化酶激酶处理该酶可更完全地克服这种抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2543/1130988/944fed89e001/biochemj00138-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2543/1130988/944fed89e001/biochemj00138-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2543/1130988/944fed89e001/biochemj00138-0046-a.jpg

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本文引用的文献

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Kinetics of activation of acetyl-CoA carboxylase by citrate. Relationship to the rate of polymerization of the enzyme.柠檬酸对乙酰辅酶A羧化酶的激活动力学。与酶聚合速率的关系。
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Coenzyme A activation of acetyl-CoA carboxylase.辅酶A对乙酰辅酶A羧化酶的激活作用。
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Regulation of acetyl-coA carboxylase: properties of coA activation of acetyl-coA carboxylase.乙酰辅酶A羧化酶的调节：辅酶A对乙酰辅酶A羧化酶的激活特性
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3351-5. doi: 10.1073/pnas.77.6.3351.
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