Pavelic Z P, Pavelic L, Lower E E, Gapany M, Gapany S, Barker E A, Preisler H D
Department of Otolaryngology-Head and Neck Surgery, College of Medicine, University of Cincinnati, Ohio 45267-0528.
Cancer Res. 1992 May 1;52(9):2597-602.
c-myc, c-erbB-2, and Ki-67 expression was examined by immunohistochemistry in 11 normal breast tissues and 42 invasive and 14 noninvasive breast carcinomas. The c-myc product was detected in all breast carcinoma specimens and in 7 of 11 normal breast tissues. Invasive tumors stained more frequently with the anti-myc monoclonal antibody than did noninvasive tumors, while the level of expression in normal breast tissue was much less than that in breast cancer. Membrane staining of the c-erbB-2 protein was demonstrated in 29% (4 of 14) of noninvasive ductal carcinomas and in 45% (19 of 42) of invasive breast carcinomas. None of the 11 normal breast tissue samples was positive. The mean value of Ki-67-positive cells was 0.91 +/- 0.31% for normal breast tissue, 4.57 +/- 1.36% for noninvasive ductal carcinoma, and 12.76 +/- 2.18% for invasive breast cancer. In 42 invasive breast carcinomas, the expression of c-myc, c-erbB-2, and Ki-67 proliferation marker were compared with lymph node status, estrogen receptor status, progesterone receptor status, and age of patients at diagnosis. c-erbB-2 overexpression and Ki-67 overexpression were identified as the only factors associated with lymph node status. We concluded that they might be additional prognostic factors for breast carcinoma.
采用免疫组织化学方法检测了11例正常乳腺组织、42例浸润性乳腺癌和14例非浸润性乳腺癌中c-myc、c-erbB-2和Ki-67的表达情况。在所有乳腺癌标本以及11例正常乳腺组织中的7例中检测到了c-myc产物。浸润性肿瘤比非浸润性肿瘤更频繁地被抗myc单克隆抗体染色，而正常乳腺组织中的表达水平远低于乳腺癌。c-erbB-2蛋白的膜染色在29%（14例中的4例）的非浸润性导管癌和45%（42例中的19例）的浸润性乳腺癌中被证实。11例正常乳腺组织样本均为阴性。正常乳腺组织中Ki-67阳性细胞的平均值为0.91±0.31%，非浸润性导管癌为4.57±1.36%，浸润性乳腺癌为12.76±2.18%。在42例浸润性乳腺癌中，比较了c-myc、c-erbB-2和Ki-67增殖标志物的表达与淋巴结状态、雌激素受体状态、孕激素受体状态以及患者诊断时的年龄。c-erbB-2过表达和Ki-67过表达被确定为与淋巴结状态相关的唯一因素。我们得出结论，它们可能是乳腺癌额外的预后因素。
