Watanabe T, Kubota Y, Sawada T, Muto T
Department of Surgery, University of Tokyo, Japan.
Dis Colon Rectum. 1992 May;35(5):488-94. doi: 10.1007/BF02049408.
To study the possible alteration of mucosal-submucosal somatostatin-containing cells in inflammatory bowel diseases (IBD), the total numbers of somatostatin-containing endocrine cells (SCEC) and submucosal ganglion cells (SGC) were counted in Crohn's disease (CD) and ulcerative colitis (UC). Tissue specimens from 25 CD and 25 UC patients were fixed in Hollande's fixative immediately after resection and were investigated by immunohistochemical staining. A single specimen was collected from 25 colorectal cancer patients, the control group. There was a significant difference in the number of SCEC between the tissues taken from the proximal colon (ascending and transverse colon) and the distal colon (descending and sigmoid colon). The distal colon tended to contain more somatostatin-immunoreactive cells than did the proximal colon. In IBD, SCEC were decreased in number compared with the controls. This decrease was related to the degree of inflammation in CD; the higher the grade of inflammation, the lower the number of SCEC. The number of SGC was decreased in IBD: however, a significant decrease was noticed only in CD. The anatomic origin and the degree of inflammation did not affect the number of SGC. In the present study, the decrease of somatostatin-containing cells was noticed in both CD and UC, but there was no significant difference between CD and UC. Therefore, it was assumed that this decrease was secondary to inflammation. However, the decrease of somatostatin, which works as an inhibitory peptide for inflammation, might have some role in the pathogenesis of IBD.
为研究炎症性肠病（IBD）中黏膜 - 黏膜下层含生长抑素细胞的可能变化，对克罗恩病（CD）和溃疡性结肠炎（UC）中含生长抑素的内分泌细胞（SCEC）总数及黏膜下层神经节细胞（SGC）进行了计数。25例CD患者和25例UC患者的组织标本在切除后立即用霍兰德固定液固定，并通过免疫组织化学染色进行研究。从25例结直肠癌患者（对照组）中采集单个标本。取自近端结肠（升结肠和横结肠）的组织与远端结肠（降结肠和乙状结肠）的组织在SCEC数量上存在显著差异。远端结肠往往比近端结肠含有更多的生长抑素免疫反应性细胞。在IBD中，与对照组相比，SCEC数量减少。这种减少与CD中的炎症程度相关；炎症分级越高，SCEC数量越低。IBD中SGC数量减少；然而，仅在CD中观察到显著减少。解剖学起源和炎症程度不影响SGC数量。在本研究中，CD和UC中均观察到含生长抑素细胞减少，但CD和UC之间无显著差异。因此，推测这种减少是炎症继发的。然而，作为炎症抑制肽的生长抑素减少可能在IBD的发病机制中起一定作用。
