胃蛋白酶和胃蛋白酶原的酶促去磷酸化作用。
Enzymic dephosphorylation of pepsin and pepsinogen.
作者信息
PERLMANN G E
出版信息
J Gen Physiol. 1958 Jan 20;41(3):441-50. doi: 10.1085/jgp.41.3.441.
It has been shown by the work presented in this paper that it is possible to dephosphorylate enzymically pepsin and pepsinogen with a variety of phosphatases. With the aid of a phosphodiesterase and the prostate phosphatase it has been established that the phosphorus in the two proteins is present as a diester and connects two sites of the peptide chain in a cyclic configuration. Removal of the phosphorus does not affect the proteolytic activity against hemoglobin or the synthetic substrate acetyl-L-phenylalanyl diiodotryosine, nor the pepsinogen pepsin transformation. However, an increase of the autodigestion of pepsin is observed.
本文所展示的工作表明，用多种磷酸酶对胃蛋白酶和胃蛋白酶原进行酶促去磷酸化是可能的。借助磷酸二酯酶和前列腺磷酸酶已确定，这两种蛋白质中的磷以二酯形式存在，并以环状结构连接肽链的两个位点。去除磷并不影响对血红蛋白或合成底物乙酰 - L - 苯丙氨酰二碘酪氨酸的蛋白水解活性，也不影响胃蛋白酶原向胃蛋白酶的转化。然而，观察到胃蛋白酶的自身消化有所增加。
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