结晶胃蛋白酶的氨基酸组成。
The amino acid composition of crystalline pepsin.
作者信息
BLUMENFELD O O, PERLMANN G E
出版信息
J Gen Physiol. 1959 Jan 20;42(3):553-61. doi: 10.1085/jgp.42.3.553.
Abstract
The amino acid composition of twice recrystallized pepsin (Worthington Biochemical Corporation) has been determined chromatographically on columns of Amberlite IR 120 resin. The results of the analyses obtained on four different preparations indicate a close agreement in their amino acid composition. Pepsin is unique in that it has a great predominance of acidic amino acids over basic ones. Moreover, all the preparations contain a small and constant amount of hydroxyproline, corresponding to about 0.1 residue per molecule.
摘要
已通过在Amberlite IR 120树脂柱上进行色谱分析，测定了两次重结晶胃蛋白酶（沃辛顿生物化学公司）的氨基酸组成。对四种不同制剂进行分析的结果表明，它们的氨基酸组成非常接近。胃蛋白酶的独特之处在于，其酸性氨基酸的含量大大超过碱性氨基酸。此外，所有制剂都含有少量且恒定的羟脯氨酸，每分子约相当于0.1个残基。
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本文引用的文献
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Electrophoresis of pepsin.胃蛋白酶的电泳
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Ultraviolet absorption spectra of proteins and amino acids.蛋白质和氨基酸的紫外吸收光谱。
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