Liau Y H, Horowitz M I
Digestion. 1976;14(4):372-5. doi: 10.1159/000197955.
The in vitro sulfation of glycoproteins, mucopolysaccharides and lipids was studied with rat stomach, duodenum and colon mucosal scrapings. In the presence of Na2 35SO4, the incorporation of label was colon greater than stomach greater than or equal to duodenum. In the presence of [35S] 3'-phosphoadenosine 5'-phosphosulfate (PAP35S), the incorporation of label was colon = stomach greater than duodenum. The 35S was incorporated into glycoprotein, mucopolysaccharide and sulfatide. It is suggested that the availability of PAPS may be an important factor in determining the differences in sulfation previously observed histochemically in several species, namely that in vivo colonic mucosa far exceeds stomach mucosa in synthesis of sulfated polyanions.
利用大鼠胃、十二指肠和结肠黏膜刮片研究了糖蛋白、黏多糖和脂质的体外硫酸化作用。在存在Na₂³⁵SO₄的情况下，标记物的掺入量为结肠＞胃≥十二指肠。在存在[³⁵S]3'-磷酸腺苷5'-磷酸硫酸酯（PAP³⁵S）的情况下，标记物的掺入量为结肠 = 胃＞十二指肠。³⁵S被掺入到糖蛋白、黏多糖和硫脂中。有人提出，PAPS的可利用性可能是决定先前在几个物种中通过组织化学观察到的硫酸化差异的一个重要因素，即在体内结肠黏膜在硫酸化多阴离子的合成方面远远超过胃黏膜。
