使用麻风分枝杆菌的18-kDa蛋白基因对分枝杆菌DNA进行DNA杂交分析。
DNA hybridization analysis of mycobacterial DNA using the 18-kDa protein gene of Mycobacterium leprae.
作者信息
Moudgil K D, Williams D L, Gillis T P
机构信息
Immunology Research Department, GWL Hansen's Disease Center, Carville, Louisiana 70721-9607.
出版信息
FEMS Microbiol Immunol. 1992 Feb;4(3):165-74. doi: 10.1111/j.1574-6968.1992.tb04983.x.
DNA hybridization studies using a 611-base pair (bp) probe, encoding the entire 18-kDa protein of Mycobacterium leprae, demonstrated that M. simiae, M. intracellulare, M. kansasii, M. terrae, ADM-2, M. avium, M. scrofulaceum, M. gordonae and M. chelonei appear to possess DNA sequences homologous to the 18-kDa protein gene of M. leprae. RFLP analysis revealed that the restriction sites in the M. leprae 18-kDa gene were not conserved in the putative gene homologs of M. simiae and M. intracellulare. The restriction patterns observed with the 611-bp probe were useful in differentiating M. intracellulare, M. simiae, and M. leprae from each other, as well as in distinguishing strains of M. simiae serovar 1. Finally, the presence of homologous sequences in various mycobacteria did not affect the specificity of a previously described PCR test for detection of M. leprae, based on the M. leprae 18-kDa protein gene.
使用编码麻风分枝杆菌完整18 kDa蛋白的611碱基对（bp）探针进行的DNA杂交研究表明，猿猴分枝杆菌、胞内分枝杆菌、堪萨斯分枝杆菌、地分枝杆菌、ADM - 2、鸟分枝杆菌、瘰疬分枝杆菌、戈登分枝杆菌和龟分枝杆菌似乎拥有与麻风分枝杆菌18 kDa蛋白基因同源的DNA序列。限制性片段长度多态性（RFLP）分析显示，麻风分枝杆菌18 kDa基因中的限制性位点在猿猴分枝杆菌和胞内分枝杆菌的假定基因同源物中并不保守。用611 bp探针观察到的限制性图谱有助于区分胞内分枝杆菌、猿猴分枝杆菌和麻风分枝杆菌，也有助于区分猿猴分枝杆菌血清型1的菌株。最后，各种分枝杆菌中同源序列的存在并不影响先前描述的基于麻风分枝杆菌18 kDa蛋白基因检测麻风分枝杆菌的PCR试验的特异性。
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