Soeharso P, Summers K M, Cooksley W G
Department of Biochemistry, University of Queensland, Australia.
Hum Genet. 1992 Apr;89(1):59-63. doi: 10.1007/BF00207043.
RFLPs of TCR beta and gamma genes have been analyzed in chronic HBV carriers of three different ethnic populations to determine if there is an association of TCR allotypes with the development of chronic hepatitis B. The RFLPs of TCR beta and gamma genes were defined respectively by BglII and PvuII genomic fragments on Southern blots. These methods allow allotype assignment. The distribution of TCR beta alleles showed ethnic variation, with one allele significantly decreased in Australian Aborigines, but there was no association with chronic hepatitis B. The distribution of TCR gamma alleles did not show ethnic variation. However, a significant frequency decrease of one allele occurred in Aboriginal HBV carriers, suggesting the possibility of involvement of TCR gamma allotypes in the development of the chronic HBV carrier state in Australian Aborigines.
已对三个不同种族群体的慢性乙肝病毒携带者的TCRβ和γ基因的限制性片段长度多态性（RFLPs）进行了分析，以确定TCR同种异型与慢性乙型肝炎的发展是否存在关联。TCRβ和γ基因的RFLPs分别通过Southern印迹上的BglII和PvuII基因组片段来定义。这些方法可进行同种异型分型。TCRβ等位基因的分布显示出种族差异，其中一个等位基因在澳大利亚原住民中显著减少，但与慢性乙型肝炎并无关联。TCRγ等位基因的分布未显示出种族差异。然而，原住民乙肝病毒携带者中一个等位基因的频率显著降低，这表明TCRγ同种异型可能参与了澳大利亚原住民慢性乙肝病毒携带状态的发展。
