Parronchi P, De Carli M, Manetti R, Simonelli C, Piccinni M P, Macchia D, Maggi E, Del Prete G, Ricci M, Romagnani S
Division of Clinical Immunology and Allergology, University of Florence, Italy.
Eur J Immunol. 1992 Jun;22(6):1615-20. doi: 10.1002/eji.1830220640.
The cytokine secretion profiles of T cell lines (TCL) specific for purified protein derivative (PPD) or streptokinase (SK), contemporarily derived from nine atopic and nine nonatopic individuals, were compared. Upon stimulation with phorbol myristate acetate (PMA) plus anti-CD3 monoclonal antibody (mAb), all TCL from both atopics and nonatopics produced interleukin (IL)-2 and interferon (IFN)-gamma. The mean IL-2 production by PPD- or SK-specific TCL from both atopics and nonatopics was similar, whereas the mean IFN-gamma production by TCL derived from atopics was significantly lower. In addition, both PPD- and SK-specific TCL from atopics produced detectable amounts of IL-4 and IL-5, whereas the corresponding TCL derived from nonatopics did not. A total number of 107 and 99 PPD-specific CD4+ T cell clones (TCC) were then derived from TCL of 4 atopic and 4 nonatopic donors and assessed for their profile of cytokine production in response to stimulation with either PMA plus anti-CD3 mAb or the specific antigen. Under both these experimental conditions, virtually all PPD-specific TCC from both atopic and nonatopic individuals produced IL-2 and IFN-gamma. In contrast, the great majority of PPD-specific TCC derived from nonatopic individuals did not produce IL-4 and IL-5, whereas high proportions of PPD-specific TCC derived from atopic donors displayed the ability to produce noticeable amounts of IL-4 and IL-5 besides IL-2 and IFN-gamma. These data indicate that CD4+ T cells from atopic individuals are able to produce IL-4 and IL-5 in response to bacterial antigens, such as PPD and SK, that usually evoke responses with a restricted type-1 T helper (Th1)-like cytokine profile in nonatopic individuals. Aberrant IL-4 production by Th cells may represent one of the immune alterations responsible for enhanced IgE antibody production in atopic people.
比较了同时来自9名特应性个体和9名非特应性个体、对纯化蛋白衍生物（PPD）或链激酶（SK）特异的T细胞系（TCL）的细胞因子分泌谱。在用佛波酯肉豆蔻酸酯乙酸盐（PMA）加抗CD3单克隆抗体（mAb）刺激后，特应性个体和非特应性个体的所有TCL均产生白细胞介素（IL）-2和干扰素（IFN）-γ。特应性个体和非特应性个体中PPD或SK特异的TCL产生IL-2的均值相似，而特应性个体来源的TCL产生IFN-γ的均值显著更低。此外，特应性个体中PPD和SK特异的TCL均产生可检测量的IL-4和IL-5，而非特应性个体来源的相应TCL则不产生。然后从4名特应性供体和4名非特应性供体的TCL中分别获得了总共107个和99个PPD特异的CD4⁺T细胞克隆（TCC），并评估了它们在用PMA加抗CD3 mAb或特异性抗原刺激后细胞因子的产生情况。在这两种实验条件下，特应性个体和非特应性个体中几乎所有PPD特异的TCC均产生IL-2和IFN-γ。相反，非特应性个体来源的绝大多数PPD特异的TCC不产生IL-4和IL-5，而特应性供体来源的高比例PPD特异的TCC除了产生IL-2和IFN-γ外，还具有产生可观量IL-4和IL-5的能力。这些数据表明，特应性个体的CD4⁺T细胞能够对细菌抗原（如PPD和SK）产生反应而分泌IL-4和IL-5，而这些抗原通常在非特应性个体中引发具有受限的1型辅助性T（Th1）样细胞因子谱的反应。Th细胞异常产生IL-4可能是导致特应性个体中IgE抗体产生增加的免疫改变之一。
