Hows J M, Bradley B A, Marsh J C, Luft T, Coutinho L, Testa N G, Dexter T M
Kay Kendall Laboratory, Paterson Institute, Cancer Research Campaign, Manchester, UK.
Lancet. 1992 Jul 11;340(8811):73-6. doi: 10.1016/0140-6736(92)90396-k.
Cryopreserved human umbilical-cord (HUC) blood is an alternative to bone marrow as a source of haemopoietic "stem" cells for HLA-identical transplantation of children with leukaemia or Fanconi's anaemia. We have studied the in-vitro growth potential of HUC blood in clonogenic assays and in longterm haemopoietic cultures. Clonogenic assays showed that HUC blood produced as many haemopoietic-cell colonies as normal adult bone marrow and a higher proportion of primitive-cell colonies. In longterm culture on preformed irradiated marrow stroma, both progenitor-cell production and lifespan of cultures were significantly greater in HUC blood than in normal bone marrow (p = 0.0007). Our findings indicate that the quality and quantity of HUC-blood-derived haemopoietic "stem" cells are better than those of normal bone marrow. Therefore, single HUC-blood donations are probably sufficient for adults requiring transplantation for leukaemia and other haemopoietic disorders. Banking of HLA-typed HUC blood to facilitate transplantation of patients who lack a family donor should be considered.
冷冻保存的人脐带血可替代骨髓，作为白血病或范科尼贫血患儿进行 HLA 配型相同移植时造血“干细胞”的来源。我们已在克隆形成试验和长期造血培养中研究了脐带血的体外生长潜力。克隆形成试验表明，脐带血产生的造血细胞集落与正常成人骨髓一样多，且原始细胞集落的比例更高。在预先照射过的骨髓基质上进行长期培养时，脐带血中祖细胞的产生量和培养物的寿命均显著高于正常骨髓（p = 0.0007）。我们的研究结果表明，脐带血来源的造血“干细胞”的质量和数量优于正常骨髓。因此，对于因白血病和其他造血系统疾病需要移植的成年人，单次脐带血捐献可能就足够了。应考虑储存 HLA 分型的脐带血，以方便为缺乏家庭供体的患者进行移植。
