Makino H, Yamasaki Y, Hironaka K, Ota Z
Third Department of Internal Medicine, Okayama University Medical School, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1992;62(1):19-24. doi: 10.1007/BF02899661.
Characteristic pathological changes in the glomeruli in diabetic nephropathy include expansion of the mesangial matrix and thickening of the glomerular basement membrane (GBM). Using an acellular digestion technique combined with scanning electron microscopy, the three-dimensional ultrastructural changes in glomerular extracellular matrices were studied in rats with diabetic glomerulopathy. Diabetes was induced by the intravenous injection of streptozotocin and morphological analyses were performed 3, 6 and 11 months after the injection. Expansion of mesangial area and GBM thickening became evident with time. After treatment with the series of detergents, all cellular components were completely removed leaving the extracellular matrices intact. In normal controls, the mesangial matrix appeared as fenestrated septa with oval or round stomata between the glomerular capillaries. In diabetic glomerulopathy, expansion of mesangial matrix and narrowing of the mesangial fenestrae were observed. These changes in the mesangial matrices seem to play a vital role in the progression of glomerulosclerosis in rat diabetes. A subendothelial thin layer of the GBM was continuous with the mesangial matrix. One cause of GBM thickening in streptozotocin diabetes may be expansion of the mesangial matrix into the peripheral GBM.
糖尿病肾病肾小球的特征性病理变化包括系膜基质扩张和肾小球基底膜（GBM）增厚。采用脱细胞消化技术结合扫描电子显微镜，研究了糖尿病肾小球病大鼠肾小球细胞外基质的三维超微结构变化。通过静脉注射链脲佐菌素诱导糖尿病，并在注射后3、6和11个月进行形态学分析。系膜面积扩张和GBM增厚随时间变得明显。用一系列去污剂处理后，所有细胞成分被完全去除，细胞外基质保持完整。在正常对照组中，系膜基质表现为有孔的隔膜，在肾小球毛细血管之间有椭圆形或圆形的小孔。在糖尿病肾小球病中，观察到系膜基质扩张和系膜小孔变窄。系膜基质的这些变化似乎在大鼠糖尿病肾小球硬化的进展中起重要作用。GBM的内皮下薄层与系膜基质连续。链脲佐菌素糖尿病中GBM增厚的一个原因可能是系膜基质向周围GBM扩张。
