Massmann G A, Figueroa J P, Hoffman G, Nathanielsz P W
Laboratory for Pregnancy and Newborn Research, New York State College of Veterinary Medicine, New York.
Biol Reprod. 1992 Mar;46(3):483-91. doi: 10.1095/biolreprod46.3.483.
The aims of the present study were to characterize the sympathetic innervation of the nonpregnant sheep uterus, to determine the catecholamine content in myometrium (MYO) and endometrium, and to study the effects of chemical sympathectomy (CHSPX) on uterine catecholamine content and on uterine electromyographic (EMG) activity recorded from the MYO and mesometrium (MESO) in the nonpregnant ovariectomized sheep. After synchronization of estrus, 9 nonpregnant sheep were anesthetized with halothane, ovariectomized, and fitted with vascular catheters and EMG electrodes. Estradiol-17 beta was administered intravascularly at a rate of 50 micrograms/24 h for 10 days. CHSPX was induced with 6-hydroxy dopamine (20 mg/kg). Uterine tissues were obtained for determination of catecholamine content by HPLC and for immunocytochemical staining using an antibody against tyrosine hydroxylase (TH). In nonpregnant ovariectomized sheep, TH immunostaining was present in nerve fibers located in endometrium and MYO. In all layers of the uterus, catecholamine fibers were found in the proximity of blood vessels as well as in defined regions of the parenchyma. Throughout the uterus, norepinephrine content and TH immunostaining were dramatically decreased after CHSPX. CHSPX decreased uterine short EMG event activity in both MYO and MESO. Contracture-type activity was not affected in MYO and was increased in MESO. We conclude that sympathetic innervation modulates the MYO and MESO EMG activity in nonpregnant ovariectomized sheep under estradiol supplementation, and that the removal of the sympathetic innervation induces a decrease in the spontaneous activity.
本研究的目的是描绘未孕绵羊子宫的交感神经支配情况，测定子宫肌层（MYO）和子宫内膜中的儿茶酚胺含量，并研究化学性交感神经切除术（CHSPX）对未孕去卵巢绵羊子宫儿茶酚胺含量以及从子宫肌层和子宫系膜（MESO）记录的子宫肌电图（EMG）活动的影响。在发情同步后，9只未孕绵羊用氟烷麻醉，进行去卵巢手术，并安装血管导管和EMG电极。以50微克/24小时的速率静脉注射17β-雌二醇，持续10天。用6-羟基多巴胺（20毫克/千克）诱导CHSPX。获取子宫组织，通过高效液相色谱法测定儿茶酚胺含量，并使用抗酪氨酸羟化酶（TH）抗体进行免疫细胞化学染色。在未孕去卵巢绵羊中，TH免疫染色存在于位于子宫内膜和子宫肌层的神经纤维中。在子宫的所有层中，在血管附近以及实质的特定区域发现了儿茶酚胺纤维。在整个子宫中，CHSPX后去甲肾上腺素含量和TH免疫染色显著降低。CHSPX降低了子宫肌层和子宫系膜中子宫短EMG事件活动。挛缩型活动在子宫肌层中未受影响，在子宫系膜中增加。我们得出结论，在补充雌二醇的情况下，交感神经支配调节未孕去卵巢绵羊的子宫肌层和子宫系膜EMG活动，并且去除交感神经支配会导致自发活动减少。
