Sarker A B, Akagi T, Jeon H J, Miyake K, Murakami I, Yoshino T, Takahashi K, Nose S
Second Department of Pathology, Okayama University Medical School, Japan.
Am J Pathol. 1992 Jul;141(1):19-23.
Thirty-three cases of Hodgkin's disease (thirteen nodular sclerosis, four diffuse, lymphocyte predominance, and sixteen mixed cellularity) were studied with Bauhinia purpurea (BPA), peanut agglutinin (PNA), anti-Leu-M1, LN2, and Ber-H2 by the avidinbiotin-peroxidase complex (ABC) method in paraffin sections. Reed-Sternberg (RS) cells and variants were stained positively with one or more of the reagents in all cases. BPA staining was positive in 32 of 33 cases (97.0%), PNA staining was positive in 23 of 33 cases (69.7%), Leu-M1 was positive in 13 of 33 cases (39.4%), LN2 was positive in 14 of 33 cases (42.4%), and Ber-H2 was positive in 24 of 33 cases (72.7%). Many RS cells were stained moderately to strongly and were readily recognized in 31 cases (96.9%) of BPA+, 10 (43.5%) of PNA+, 8 (61.5%) of Leu-M1+, 6 (42.9%) of LN2+, and 22 (91.7%) of Ber-H2+ cases; in the remaining positive cases, the RS cells were found only after careful searching. Three staining patterns were recognized: paranuclear, diffuse cytoplasmic, and membranous. These three patterns were obtained with all markers except for LN2. LN2 showed diffuse cytoplasmic staining in most of the positive cells, and a few cells showed paranuclear deposits. BPA reactivity was not affected by formalin fixation or paraffin embedding. Except for RS cells, BPA also showed dense cytoplasmic staining reaction with macrophage-histiocytes. Sixty cases of non-Hodgkin's diffuse lymphomas (30 T- and 30 B-cell origin) were also studied. Tumor cells were not stained with BPA, PNA, and Leu-M1, but stained positively with LN2 in six T-cell lymphomas and thirteen B-cell lymphomas, and with Ber-H2 in six T-cell lymphomas and one B-cell lymphoma. In conclusion, to facilitate the detection of RS cells and related variants in paraffin sections, BPA can be accepted as a useful marker due to its high-detection rate, reproducible staining pattern, and resistance to fixatives.
采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，在石蜡切片中用紫羊蹄甲（BPA）、花生凝集素（PNA）、抗Leu-M1、LN2和Ber-H2对33例霍奇金病（13例结节硬化型、4例弥漫性淋巴细胞为主型和16例混合细胞型）进行研究。在所有病例中，里德-施特恩伯格（RS）细胞及其变异型均被一种或多种试剂阳性染色。33例中有32例（97.0%）BPA染色阳性，33例中有23例（69.7%）PNA染色阳性，33例中有13例（39.4%）Leu-M1阳性，33例中有14例（42.4%）LN2阳性，33例中有24例（72.7%）Ber-H2阳性。许多RS细胞呈中度至强阳性染色，在31例（96.9%）BPA阳性、10例（43.5%）PNA阳性、8例（61.5%）Leu-M1阳性、6例（42.9%）LN2阳性和22例（91.7%）Ber-H2阳性病例中易于识别；在其余阳性病例中，只有经过仔细查找才能发现RS细胞。识别出三种染色模式：核旁、弥漫性胞质和膜性。除LN2外，所有标志物均呈现这三种模式。LN2在大多数阳性细胞中呈弥漫性胞质染色，少数细胞呈核旁沉积。BPA反应性不受福尔马林固定或石蜡包埋的影响。除RS细胞外，BPA还与巨噬细胞-组织细胞呈现致密的胞质染色反应。还对60例非霍奇金弥漫性淋巴瘤（30例T细胞起源和30例B细胞起源）进行了研究。肿瘤细胞不被BPA、PNA和Leu-M1染色，但在6例T细胞淋巴瘤和13例B细胞淋巴瘤中被LN2阳性染色，在6例T细胞淋巴瘤和1例B细胞淋巴瘤中被Ber-H2阳性染色。总之，为便于在石蜡切片中检测RS细胞及其相关变异型，由于BPA检测率高、染色模式可重复且对固定剂有抗性，可将其作为一种有用的标志物。
