Weller M, Esser P, Bresgen M, Heimann K, Wiedemann P
Department of Vitreoretinal Surgery, University Eye Clinic, Cologne, Germany.
Eur J Ophthalmol. 1992 Jan-Mar;2(1):10-4. doi: 10.1177/112067219200200103.
Thrombospondin (TSP), an adhesive integrin-binding protein of plasma and platelets, was detected in preretinal traction membranes from patients with idiopathic (8/8) and traumatic (7/8) proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR) (6/8). TSP immunoreactivity was compared to the pattern of von Willebrand factor, plasma transglutaminase (blood coagulation factor XIII), fibronectin, and mononuclear phagocytes, using double-label immunofluorescence microscopy. TSP was partially colocalised with the endothelial cell marker, von Willebrand factor, in PDR. The codistribution of catalytic factor XIII and two cross-linking substrates, fibronectin and TSP, suggests a functional role of the enzyme in the extracellular matrix build-up in PVR and PDR. No significant TSP synthesis by mononuclear phagocytes was observed. Western blotting indicated a plasmin-mediated intravitreal breakdown of presumably plasmatic TSP in PVR and PDR.
血小板反应蛋白（TSP）是一种存在于血浆和血小板中的黏附性整合素结合蛋白，在特发性（8/8）和外伤性（7/8）增殖性玻璃体视网膜病变（PVR）以及增殖性糖尿病视网膜病变（PDR）（6/8）患者的视网膜前牵引膜中被检测到。使用双标记免疫荧光显微镜，将TSP免疫反应性与血管性血友病因子、血浆转谷氨酰胺酶（凝血因子XIII）、纤连蛋白和单核吞噬细胞的模式进行了比较。在PDR中，TSP与内皮细胞标志物血管性血友病因子部分共定位。催化因子XIII与两种交联底物纤连蛋白和TSP的共分布表明该酶在PVR和PDR的细胞外基质形成中起功能性作用。未观察到单核吞噬细胞有明显的TSP合成。蛋白质印迹法表明，在PVR和PDR中，纤溶酶介导了玻璃体中可能来自血浆的TSP的分解。
