Esser P, Weller M, Heimann K, Wiedemann P
Abteilung für Netzhaut und Glaskörperchirurgie, Universitäts-Augenklinik, Köln, Bundesrepublik Deutschland.
Fortschr Ophthalmol. 1991;88(4):337-40.
Thrombospondin (TSP), a platelet-derived protein of the integrin-binding family with adhesive and mitogenic properties was localized in surgically obtained epiretinal traction membranes from patients with traumatic (7/8) and idiopathic (8/8) proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR) (6/8). Using double-label immunofluorescence techniques, we demonstrated co-localization of TSP with the endothelial cell marker, von Willebrand factor, in PDR; however, only a minority of labeled macrophages showed simultaneous staining for TSP. Therefore, macrophages are probably not a major source of TSP in PVR. We demonstrated co-distribution of blood coagulation factor XIII and two of its cross-linking substrates, fibronectin and TSP, in epiretinal membranes, as well as the detection of plasmin and presumably plasmin-induced TSP breakdown products in physiologic and pathologic vitreous. These results suggest that the coagulation system has a functional role in proliferative retinal disorders and imply that the application of inhibitors of the coagulation cascade like heparin may be a potential therapeutic approach.
血小板反应蛋白（TSP）是整合素结合家族中一种具有黏附及促有丝分裂特性的血小板衍生蛋白，在因创伤性增殖性玻璃体视网膜病变（PVR，7/8例）、特发性增殖性玻璃体视网膜病变（8/8例）和增殖性糖尿病视网膜病变（PDR，6/8例）而通过手术获取的视网膜前牵引膜中被定位。运用双标记免疫荧光技术，我们证实在PDR中TSP与内皮细胞标志物血管性血友病因子共定位；然而，仅有少数标记的巨噬细胞显示同时被TSP染色。因此，巨噬细胞可能不是PVR中TSP的主要来源。我们证实在视网膜前膜中凝血因子XIII及其两种交联底物纤连蛋白和TSP共同分布，以及在生理和病理玻璃体中检测到纤溶酶和推测的纤溶酶诱导的TSP降解产物。这些结果表明凝血系统在增殖性视网膜疾病中起功能性作用，并提示应用像肝素这样的凝血级联抑制剂可能是一种潜在的治疗方法。
