Virji M, Alexandrescu C, Ferguson D J, Saunders J R, Moxon E R
Department of Paediatrics, University of Oxford, John Radcliffe Hospital, UK.
Mol Microbiol. 1992 May;6(10):1271-9. doi: 10.1111/j.1365-2958.1992.tb00848.x.
The effect of variations in Neisseria meningitidis pili on bacterial interactions with three epithelial cell lines as well as human umbilical vein endothelial cells was studied using a panel of seven strains expressing Class I or Class II pili. Comparison of adherence of piliated and pilus-deficient variants of each strain to epithelial cells suggested that Class I pili may mediate bacterial adherence with all three epithelial cell lines. In contrast, Class II pili of the strains used did not increase bacterial adherence to Hep-2 larynx carcinoma cells, although an increase in adherence to Chang conjunctival and A549 lung carcinoma epithelial cells was observed in the Class II pili-expressing strains. In addition to these interclass functional variations, differences in adherence to epithelial cells were also observed among Class I and Class II strains. Functionally different pilin variants of one Class I strain, MC58, were obtained by single colony isolation. One piliated variant was identified which had concurrently lost the ability to adhere to both Chang and Hep-2 cells ('non-adherent' phenotype; adherence of less than 2 bacteria per cell). In addition, several adherent pilin variants were isolated from non-adherent Pil- and Pil+ bacteria by selection on Chang cells (adherence of 10-25 bacteria per cell). In contrast to epithelial cells, all variant pili, whether of Class I or Class II, adhered to endothelial cells in substantially larger numbers (greater than 50 bacteria per cell) and therefore implied the existence of distinct mechanisms in pilus-facilitated interactions of N. meningitidis with endothelial and epithelial cells.
使用一组表达I类或II类菌毛的七株菌株，研究了脑膜炎奈瑟菌菌毛变异对细菌与三种上皮细胞系以及人脐静脉内皮细胞相互作用的影响。比较各菌株的菌毛化和菌毛缺陷变体对上皮细胞的黏附情况表明，I类菌毛可能介导细菌与所有三种上皮细胞系的黏附。相比之下，所用菌株的II类菌毛虽未增加对Hep-2喉癌细胞的细菌黏附，但在表达II类菌毛的菌株中，观察到对Chang结膜细胞和A549肺癌上皮细胞的黏附增加。除了这些类间功能差异外，I类和II类菌株之间在上皮细胞黏附方面也存在差异。通过单菌落分离获得了I类菌株MC58的功能不同的菌毛蛋白变体。鉴定出一种菌毛化变体，它同时丧失了黏附Chang细胞和Hep-2细胞的能力（“非黏附”表型；每个细胞黏附细菌少于2个）。此外，通过在Chang细胞上筛选，从非黏附的Pil-和Pil+细菌中分离出了几种黏附性菌毛蛋白变体（每个细胞黏附细菌10 - 25个）。与上皮细胞不同，所有变体菌毛，无论是I类还是II类，都以大量数量（每个细胞大于50个细菌）黏附于内皮细胞，因此表明脑膜炎奈瑟菌菌毛促进的与内皮细胞和上皮细胞相互作用存在不同机制。
