Batenburg J J, Elfring R H
Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.
FEBS Lett. 1992 Jul 28;307(2):164-8. doi: 10.1016/0014-5793(92)80759-a.
Exposure to fibroblast-conditioned cortisol-containing medium increased fatty acid synthase activity and fatty acid synthase, acetyl-CoA carboxylase and ATP citrate lyase mRNA abundance in fetal type II alveolar epithelial cells. Both fibroblast conditioning and cortisol in the medium were required for maximal effect on the mRNA levels, indicating involvement of mesenchymal-epithelial interaction in the cortisol effects. The observed effects provide evidence for an earlier hypothesis that increased activity of CTP:phosphocholine cytidylyltransferase in lung tissue caused by glucocorticoid is due to increased fatty acid synthesis. However, evidence suggesting pre-translational regulation of this enzyme by glucocorticoid was also found.
暴露于成纤维细胞条件培养的含皮质醇培养基中，可增加胎儿II型肺泡上皮细胞中的脂肪酸合酶活性以及脂肪酸合酶、乙酰辅酶A羧化酶和ATP柠檬酸裂解酶的mRNA丰度。培养基中的成纤维细胞条件培养和皮质醇对于mRNA水平产生最大影响均是必需的，这表明间充质-上皮相互作用参与了皮质醇的作用。观察到的这些效应为早期的一个假说提供了证据，即糖皮质激素导致肺组织中CTP：磷酸胆碱胞苷转移酶活性增加是由于脂肪酸合成增加所致。然而，也发现了糖皮质激素对该酶进行翻译前调控的证据。
