Kim K S, Park S W, Moon Y A, Kim Y S
Department of Biochemistry, Yonsei University College of Medicine, Seoul, Korea.
Biochem J. 1994 Sep 15;302 ( Pt 3)(Pt 3):759-64. doi: 10.1042/bj3020759.
A genomic clone, encompassing the 5' flanking region and the first seven exons of rat ATP citrate lyase gene, was isolated from a rat genomic library and sequenced. Primer-extension analysis showed that mRNA is transcribed at 4407 nucleotides upstream from the translation start site. Primer-extension analysis and sequencing of ATP citrate lyase cDNA amplified by PCR showed that the promoter used for transcription is identical in mammary gland, lung, liver, brain and kidney. Southern-blot analysis showed that the ATP citrate lyase gene exists as a single copy. The 5' flanking region contains several consensus sequences defined as promoter elements. These include a CAAT box and Sp1-binding sites. However, a TATA box lacks this promoter. The expression of the chloramphenicol acetyltransferase gene was induced by the 5' flanking region (-2370 to -1) in the CHO cell line. The 5' flanking region also contains several sequence elements that may be involved in the transcriptional regulation of the gene.
从大鼠基因组文库中分离出一个包含大鼠ATP柠檬酸裂解酶基因5'侧翼区和前七个外显子的基因组克隆并进行测序。引物延伸分析表明，mRNA在翻译起始位点上游4407个核苷酸处转录。通过PCR扩增的ATP柠檬酸裂解酶cDNA的引物延伸分析和测序表明，用于转录的启动子在乳腺、肺、肝、脑和肾中是相同的。Southern印迹分析表明，ATP柠檬酸裂解酶基因以单拷贝形式存在。5'侧翼区包含几个被定义为启动子元件的共有序列。这些包括一个CAAT盒和Sp1结合位点。然而，这个启动子缺乏TATA盒。氯霉素乙酰转移酶基因的表达在CHO细胞系中被5'侧翼区（-2370至-1）诱导。5'侧翼区还包含几个可能参与该基因转录调控的序列元件。
