• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Activation of the mouse proliferating cell nuclear antigen gene promoter by adenovirus type 12 E1A proteins.12型腺病毒E1A蛋白对小鼠增殖细胞核抗原基因启动子的激活作用
Jpn J Cancer Res. 1992 Jun;83(6):609-17. doi: 10.1111/j.1349-7006.1992.tb00133.x.
2
The adenovirus E1A transforming protein activates the proliferating cell nuclear antigen promoter via an activating transcription factor site.腺病毒E1A转化蛋白通过一个激活转录因子位点激活增殖细胞核抗原启动子。
J Virol. 1991 Dec;65(12):6397-406. doi: 10.1128/JVI.65.12.6397-6406.1991.
3
Activation of the mouse DNA polymerase beta gene promoter by adenovirus type 12 E1A proteins.12型腺病毒E1A蛋白对小鼠DNA聚合酶β基因启动子的激活作用。
Nucleic Acids Res. 1992 May 11;20(9):2321-5. doi: 10.1093/nar/20.9.2321.
4
Adenovirus early region 3 promoter regulation by E1A/E1B is independent of alterations in DNA binding and gene activation of CREB/ATF and AP1.腺病毒早期区域3启动子受E1A/E1B的调控独立于CREB/ATF和AP1的DNA结合及基因激活的改变。
J Virol. 1990 May;64(5):2004-13. doi: 10.1128/JVI.64.5.2004-2013.1990.
5
The human cytomegalovirus immediate early enhancer-promoter is responsive to activation by the adenovirus-5 13S E1A gene.人巨细胞病毒立即早期增强子-启动子对腺病毒5型13S E1A基因的激活有反应。
Arch Virol. 1990;112(1-2):67-80. doi: 10.1007/BF01348986.
6
A complex promoter element mediates transactivation of the human proliferating cell nuclear antigen promoter by the 243-residue adenovirus E1A oncoprotein.一个复杂的启动子元件介导243个氨基酸残基的腺病毒E1A癌蛋白对人增殖细胞核抗原启动子的反式激活作用。
Mol Cell Biol. 1993 Mar;13(3):1697-707. doi: 10.1128/mcb.13.3.1697-1707.1993.
7
Modulation of transcriptional activation of the proliferating cell nuclear antigen promoter by the adenovirus E1A 243-residue oncoprotein depends on proximal activators.腺病毒E1A 243个氨基酸残基的癌蛋白对增殖细胞核抗原启动子转录激活的调节取决于近端激活因子。
Mol Cell Biol. 1994 Jan;14(1):543-53. doi: 10.1128/mcb.14.1.543-553.1994.
8
Functional diversity of E1A gene autoregulation among human adenoviruses.人类腺病毒中E1A基因自动调节的功能多样性
J Virol. 1992 Jun;66(6):3833-45. doi: 10.1128/JVI.66.6.3833-3845.1992.
9
Transcriptional coactivator cAMP response element binding protein mediates induction of the human proliferating cell nuclear antigen promoter by the adenovirus E1A oncoprotein.转录共激活因子环磷酸腺苷反应元件结合蛋白介导腺病毒E1A癌蛋白对人增殖细胞核抗原启动子的诱导作用。
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4481-6. doi: 10.1073/pnas.94.9.4481.
10
Multiple transcription factor binding sites mediate adenovirus E1A transactivation.多个转录因子结合位点介导腺病毒E1A反式激活。
J Virol. 1989 Aug;63(8):3499-506. doi: 10.1128/JVI.63.8.3499-3506.1989.

引用本文的文献

1
Transcription factor decoy oligonucleotide-based therapeutic strategy for renal disease.基于转录因子诱饵寡核苷酸的肾脏疾病治疗策略
Clin Exp Nephrol. 2007 Mar;11(1):7-17. doi: 10.1007/s10157-007-0459-6. Epub 2007 Mar 28.
2
Inhibition of mesangial cell proliferation by E2F decoy oligodeoxynucleotide in vitro and in vivo.E2F诱饵寡脱氧核苷酸在体外和体内对系膜细胞增殖的抑制作用。
J Clin Invest. 1998 Jun 1;101(11):2589-97. doi: 10.1172/JCI429.
3
Characterization of the nonmuscle myosin heavy chain IIB promoter: regulation by E2F.非肌肉肌球蛋白重链IIB启动子的特性：受E2F调控
Gene Expr. 1996;6(1):45-57.
4
MCB elements and the regulation of DNA replication genes in yeast.酵母中的MCB元件与DNA复制基因的调控
Curr Genet. 1993 Sep;24(3):185-92. doi: 10.1007/BF00351790.
5
Transcriptional regulation of DNA replication-related genes in cell growth, differentiation and oncogenesis.细胞生长、分化和肿瘤发生过程中DNA复制相关基因的转录调控。
Jpn J Cancer Res. 1994 Jan;85(1):1-8. doi: 10.1111/j.1349-7006.1994.tb02878.x.
6
Cellular targets for activation by the E2F1 transcription factor include DNA synthesis- and G1/S-regulatory genes.E2F1转录因子激活的细胞靶点包括DNA合成及G1/S调控基因。
Mol Cell Biol. 1995 Aug;15(8):4215-24. doi: 10.1128/MCB.15.8.4215.
7
A gene therapy strategy using a transcription factor decoy of the E2F binding site inhibits smooth muscle proliferation in vivo.一种使用E2F结合位点转录因子诱饵的基因治疗策略可在体内抑制平滑肌增殖。
Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):5855-9. doi: 10.1073/pnas.92.13.5855.

本文引用的文献

1
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
2
Expression of adenovirus type 12 early region 1 in KB cells transformed by recombinants containing the gene.12型腺病毒早期区域1在含该基因的重组体转化的KB细胞中的表达
J Virol. 1983 Mar;45(3):1074-82. doi: 10.1128/JVI.45.3.1074-1082.1983.
3
A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.一种淋巴细胞特异性细胞增强子位于免疫球蛋白重链基因连接区的下游。
Cell. 1983 Jul;33(3):729-40. doi: 10.1016/0092-8674(83)90015-6.
4
Activation of gene expression by adenovirus and herpesvirus regulatory genes acting in trans and by a cis-acting adenovirus enhancer element.腺病毒和疱疹病毒的反式作用调控基因以及顺式作用的腺病毒增强子元件对基因表达的激活作用。
Cell. 1983 Nov;35(1):127-36. doi: 10.1016/0092-8674(83)90215-5.
5
Genetic dissection of neural properties using somatic cell hybrids.利用体细胞杂种对神经特性进行遗传剖析。
Nat New Biol. 1972 Feb 23;235(60):225-31. doi: 10.1038/newbio235225a0.
6
Transcription factor Sp1 recognizes a DNA sequence in the mouse dihydrofolate reductase promoter.转录因子Sp1识别小鼠二氢叶酸还原酶启动子中的一个DNA序列。
Nature. 1986;319(6050):246-8. doi: 10.1038/319246a0.
7
An enhancer-like element in the adenovirus E2 promoter contains sequences essential for uninduced and E1A-induced transcription.腺病毒E2启动子中的一个类增强子元件含有未诱导和E1A诱导转录所必需的序列。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):381-5. doi: 10.1073/pnas.82.2.381.
8
Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.反式作用蛋白因子与真核转录调控：来自DNA肿瘤病毒研究的经验教训
Genes Dev. 1988 Mar;2(3):267-81. doi: 10.1101/gad.2.3.267.
9
Human beta-polymerase gene. Structure of the 5'-flanking region and active promoter.人类β聚合酶基因。5'侧翼区域的结构及活性启动子。
J Biol Chem. 1988 Nov 15;263(32):16992-8.
10
Activation of the human beta interferon gene by the adenovirus type 12 E1B gene.腺病毒12型E1B基因对人β干扰素基因的激活作用。
J Virol. 1988 Jan;62(1):325-30. doi: 10.1128/JVI.62.1.325-330.1988.

12型腺病毒E1A蛋白对小鼠增殖细胞核抗原基因启动子的激活作用

Activation of the mouse proliferating cell nuclear antigen gene promoter by adenovirus type 12 E1A proteins.

作者信息

Yamaguchi M, Hayashi Y, Hirose F, Matsuoka S, Shiroki K, Matsukage A

机构信息

Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya.

出版信息

Jpn J Cancer Res. 1992 Jun;83(6):609-17. doi: 10.1111/j.1349-7006.1992.tb00133.x.

DOI:10.1111/j.1349-7006.1992.tb00133.x
PMID:1353754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5918878/
Abstract

A plasmid carrying the 5'-flanking region (-1584 to +47 with respect to the transcription initiation site) of the mouse proliferating cell nuclear antigen (PCNA) gene was fused with the chloramphenicol acetyltransferase (CAT) gene, and then cotransfected into mouse N18TG2 cells with expression plasmids for the adenovirus type 12 E1 genes. Expression of E1A gene products elevated the CAT expression by 5- to 9-fold, but expression of the E1B gene product did not. RNase protection analysis revealed that the activation of the PCNA gene promoter by E1A was at the transcription step. Both the 13S E1A and the 12S E1A activated the PCNA gene promoter, indicating that the activation domain of E1A resides in a common region(s) of 13S and 12S E1A products. The major target region of E1A was mapped within the 68 base-pair region (-21 to +47) of the PCNA gene, which includes consensus sequences for transcription factors PEA3 and E2F, although the upstream region (-83 to -21) including ATF(CREB)-binding consensus had an additional effect in the transactivation.

摘要

将携带小鼠增殖细胞核抗原（PCNA）基因5'-侧翼区（相对于转录起始位点为-1584至+47）的质粒与氯霉素乙酰转移酶（CAT）基因融合，然后与12型腺病毒E1基因的表达质粒共转染到小鼠N18TG2细胞中。E1A基因产物的表达使CAT表达提高了5至9倍，但E1B基因产物的表达则没有。核糖核酸酶保护分析表明，E1A对PCNA基因启动子的激活发生在转录步骤。13S E1A和12S E1A均激活了PCNA基因启动子，这表明E1A的激活结构域位于13S和12S E1A产物的共同区域。E1A的主要靶区域定位于PCNA基因的68个碱基对区域（-21至+47）内，该区域包含转录因子PEA3和E2F的共有序列，尽管包括ATF（CREB）结合共有序列的上游区域（-83至-21）在反式激活中具有额外作用。