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腺病毒12型E1B基因对人β干扰素基因的激活作用。

Activation of the human beta interferon gene by the adenovirus type 12 E1B gene.

作者信息

Shiroki K, Toth M

机构信息

Institute of Medical Science, University of Tokyo, Japan.

出版信息

J Virol. 1988 Jan;62(1):325-30. doi: 10.1128/JVI.62.1.325-330.1988.

DOI:10.1128/JVI.62.1.325-330.1988
PMID:2960835
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC250533/
Abstract

The transcription of endogenous beta interferon mRNA was activated in human embryo kidney (HEK) cells infected with adenovirus type 12 (Ad12) but was activated only inefficiently or not at all in HEK cells infected with Ad5 and rc-1 (Ad5 dl312 containing the Ad12 E1A region). The analysis with Ad12 mutants showed that Ad12 E1B products, especially the 19K protein, were important for the expression of the endogenous beta interferon gene and Ad12 E1A products were not involved in the expression. The expression of exogenously transfected pIFN-CAT (a hybrid plasmid having the human beta interferon promoter fused with the CAT gene) was activated in HEK and chicken embryo fibroblast (CEF) cells infected with either Ad12 or Ad5. The analysis of cotransfection of CEF cells with pIFN-CAT and plasmids containing fragments of Ad12 or Ad5 DNA showed that Ad12 or Ad5 E1B (possibly the 19K protein) was and E1A was not involved in the expression of the exogenous pIFN-CAT.

摘要

在感染12型腺病毒（Ad12）的人胚肾（HEK）细胞中，内源性β干扰素mRNA的转录被激活，但在感染Ad5和rc - 1（含有Ad12 E1A区域的Ad5 dl312）的HEK细胞中，其转录仅被低效激活或根本未被激活。对Ad12突变体的分析表明，Ad12 E1B产物，尤其是19K蛋白，对内源性β干扰素基因的表达很重要，而Ad12 E1A产物不参与该表达。在感染Ad12或Ad5的HEK细胞和鸡胚成纤维细胞（CEF）中，外源性转染的pIFN - CAT（一种将人β干扰素启动子与CAT基因融合的杂交质粒）的表达被激活。用pIFN - CAT与含有Ad12或Ad5 DNA片段的质粒共转染CEF细胞的分析表明，Ad12或Ad5 E1B（可能是19K蛋白）参与且E1A不参与外源性pIFN - CAT的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/da130e519a54/jvirol00080-0349-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/5ad16f401735/jvirol00080-0347-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/9eebea1ce966/jvirol00080-0347-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/091adee48dce/jvirol00080-0348-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/53d3d66ab1a9/jvirol00080-0349-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/5ad16f401735/jvirol00080-0347-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/9eebea1ce966/jvirol00080-0347-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/96ab4ff16f2b/jvirol00080-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/e796451c7140/jvirol00080-0348-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/815f/250533/53d3d66ab1a9/jvirol00080-0349-a.jpg
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本文引用的文献

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Structure of two adenovirus type 12 transforming polypeptides and their evolutionary implications.12型腺病毒两种转化多肽的结构及其进化意义
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
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Isolation of a nondefective recombinant between adenovirus type 5 and early region 1A of adenovirus type 12.5型腺病毒与12型腺病毒早期区域1A之间非缺陷重组体的分离
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Expression of adenovirus type 12 early region 1 in KB cells transformed by recombinants containing the gene.12型腺病毒早期区域1在含该基因的重组体转化的KB细胞中的表达
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