Gruia-Gray J, Desser S S
Department of Zoology, University of Toronto, Ont., Canada.
Biochem Cell Biol. 1992 Feb;70(2):123-8. doi: 10.1139/o92-018.
Erythrocytes of bullfrogs (Rana catesbeiana) infected with frog erythrocytic virus are spheroid and their nucleus is displaced. In contrast, uninfected cells are ellipsoid and have a centralized nucleus. Fluorescent staining revealed that these changes are correlated with alterations to components of the erythrocyte cytoskeleton. Uninfected erythrocytes contained a broad, continuous marginal band of microtubules, which appeared thinner and interrupted in infected cells. The described disruption of microtubules was associated with an inability to polymerize the tubulin pool with the addition of 12 microM taxol. The arrangement of submembranous microfilaments in uninfected erythrocytes was not significantly altered in infected cells. Vimentin filaments were distributed throughout the cytoplasm and around the nucleus of uninfected cells, and concentrated at the cell and nuclear peripheries. Cytoplasmic pockets that did not contain vimentin filaments were associated with the viral assembly site(s) in infected cells. These data suggest that the distortion of viral-infected erythrocytes could be due, in part, to an irreversible depolymerization of microtubules of the marginal band and a reorganization of the vimentin filament network.
感染蛙红细胞病毒的牛蛙（牛蛙属）红细胞呈球形，细胞核移位。相比之下，未感染的细胞呈椭圆形，细胞核位于中央。荧光染色显示，这些变化与红细胞细胞骨架成分的改变有关。未感染的红细胞含有一条宽阔、连续的微管边缘带，在感染的细胞中这条带显得更细且有中断。所描述的微管破坏与添加12微摩尔紫杉醇后微管蛋白库无法聚合有关。未感染红细胞中膜下微丝的排列在感染细胞中没有明显改变。波形蛋白丝分布在未感染细胞的整个细胞质和细胞核周围，并集中在细胞和细胞核周边。在感染细胞中，不含波形蛋白丝的细胞质小窝与病毒装配位点相关。这些数据表明，病毒感染红细胞的变形可能部分归因于边缘带微管的不可逆解聚和波形蛋白丝网络的重新组织。
