Howard J M, Eckert B S, Bourguignon L Y
J Gen Virol. 1983 Nov;64 (Pt 11):2379-85. doi: 10.1099/0022-1317-64-11-2379.
The organization of vimentin filaments, keratin filaments, microtubules and microfilaments was compared in canine distemper virus (CDV)-infected and uninfected cells by indirect immunofluorescence. Infection of tissue culture cells with CDV caused a total reorganization of all the cytoskeletal structures with the most notable changes in the microtubules and intermediate filaments. During virus infection two different patterns of staining were observed for both the intermediate filaments and microtubules, suggesting a step-by-step reorganization of the structures. While the two types of intermediate filaments (vimentin and keratin) had quite different staining patterns, the vimentin (but not keratin) filaments had a distribution pattern similar to the microtubules in both infected and uninfected cells. These results suggest that microtubules and vimentin (but not keratin) filaments may have a close association in CDV-infected cells.
通过间接免疫荧光法，比较了犬瘟热病毒（CDV）感染细胞和未感染细胞中波形蛋白丝、角蛋白丝、微管和微丝的组织情况。用CDV感染组织培养细胞会导致所有细胞骨架结构的完全重组，其中微管和中间丝的变化最为显著。在病毒感染期间，中间丝和微管均观察到两种不同的染色模式，这表明结构在逐步重组。虽然两种类型的中间丝（波形蛋白和角蛋白）具有截然不同的染色模式，但波形蛋白丝（而非角蛋白丝）在感染和未感染的细胞中均具有与微管相似的分布模式。这些结果表明，在CDV感染的细胞中，微管和波形蛋白丝（而非角蛋白丝）可能存在密切关联。
