Fahlbusch B, Gutsche W, Zschiesche W
Allerg Immunol (Leipz). 1976;22(1):93-6.
For the purification and further characterisation of the skin-reactive factor (SRF) of the mouse, the SRF-containing ascitic fluid of Ehrlich ascites tumor was exposed to different separation or elution procedures. After preliminary purification by (NH4)2SO4 precipitation when the total activity was not in the dialysable supernatant, gel-chromatographic separation (Sephadex G-200), immune electrophoretic investigations as well as ion exchange chromatography were performed. The skin-reactive activity in fraction I of gel chromatiography is presumably caused by a toxic substance with skin-irritating properties. The SRF demonstrable in fraction II/2 has a molecular weight similar to that of albumin, can be found in the ion exchange chromatography (DEAE-Sephadex A-50-column) in fraction III, and is of proteinic nature.
为了对小鼠皮肤反应因子（SRF）进行纯化及进一步表征，将含有SRF的艾氏腹水瘤腹水暴露于不同的分离或洗脱程序。通过硫酸铵沉淀进行初步纯化后，当总活性不在可透析的上清液中时，进行凝胶色谱分离（葡聚糖凝胶G-200）、免疫电泳研究以及离子交换色谱分析。凝胶色谱第一部分中的皮肤反应活性可能是由具有皮肤刺激性的有毒物质引起的。在第二部分/2中可检测到的SRF分子量与白蛋白相似，在离子交换色谱（二乙氨基乙基葡聚糖凝胶A-50柱）的第三部分中可发现，并且具有蛋白质性质。
