Nimberg R B, Humphries D E, Lloyd W S, Badger A M, Cooperband S R, Wells H, Schmid K
Cancer Res. 1978 Jul;38(7):1983-9.
A protein fraction that induces the resorption of bone explants in organ culture was isolated from the ascitic fluid of patients with advanced cancer metastatic to the peritoneal cavity. Partial purification was achieved by means of gel filtration, affinity chromatography, and ion-exchange chromatography. The isolated fraction, the components of which have an apparent molecular weight of 60,000, was found to be heterogeneous by disc gel electrophoresis and to be composed primarily of proteins with relatively acidic electrophoretic properties. The specific bone-resorptive activity of this protein fraction was greatly increased over that of the unfractionated starting material, and the activity could be completely destroyed upon incubation with pronase and on heating. As determined by immunoassay and extraction procedures with various solvents, the bone-resorptive action of the isolated fraction was not attributable to the presence of parathyroid hormone, prostaglandin E2 or vitamin D-like sterols. In parallel experiments the supernatants of phytohemagglutinin-stimulated normal human peripheral leukocytes were subjected to identical chromatographic techniques, and a proten fraction with a molecular weight of 60,000, which resembled the resorptive fraction isolated from cancer ascites fluid and which contained significant bone-resorptive activity, was also partially purified.
从已转移至腹膜腔的晚期癌症患者的腹水中分离出一种能在器官培养中诱导骨外植体吸收的蛋白质组分。通过凝胶过滤、亲和色谱和离子交换色谱法实现了部分纯化。分离出的组分经圆盘凝胶电泳显示具有异质性，其组分的表观分子量为60,000，主要由具有相对酸性电泳特性的蛋白质组成。该蛋白质组分的特异性骨吸收活性比未分级的起始材料有极大提高，且该活性在与链霉蛋白酶一起温育及加热后可被完全破坏。通过免疫测定和用各种溶剂的提取程序确定，分离出的组分的骨吸收作用并非归因于甲状旁腺激素、前列腺素E2或维生素D样固醇的存在。在平行实验中，用相同的色谱技术处理植物血凝素刺激的正常人外周血白细胞的上清液，一种分子量为60,000的蛋白质组分也被部分纯化，该组分类似于从癌症腹水中分离出的吸收组分并具有显著的骨吸收活性。
