Riley H D, Lau A F, Humphreys T
Cancer Research Center, Honolulu, Hawaii 96813.
Hum Hered. 1992;42(4):209-15. doi: 10.1159/000154072.
The relative genetic diversity of selected proto-oncogenes in the native Hawaiian gene pool was examined by comparing the restriction fragment length polymorphisms of these genes in a group of 23 individuals with at least part Hawaiian ancestry, and in 20 individuals from other ethnic groups. Twenty-one combinations of the proto-oncogenes, c-fms, c-myc, L-myc, c-Ha-ras, and c-Ki-ras, tested with 1 or more of the restriction enzymes Bam HI, Eco RI, Hind III, Pst I, Pvu I and Kpn I were examined. Sixteen of these did not exhibit RFLPs in Hawaiians or in other ethnic groups. Four of the combinations exhibiting RFLPs in native Hawaiians exhibited similar-sized restriction fragments in the other ethnic groups. Only in the case of c-myc digested with Pst I were 5 individuals of Hawaiian ancestry found to have an RFLP which has not been detected in other ethnic groups. These 5 cases exhibited a 13-kb c-myc fragment in addition to the 5.5-kb fragment found in most Hawaiians and always present in other ethnic groups. The presence in Hawaiians of most RFLPs found in other ethnic groups indicates that the genetic diversity of proto-oncogenes in the gene pool of native Hawaiians is not substantially less than that of other ethnic groups.
通过比较一组至少有部分夏威夷血统的23名个体以及20名其他种族个体中这些基因的限制性片段长度多态性，研究了夏威夷本土基因库中所选原癌基因的相对遗传多样性。对原癌基因c-fms、c-myc、L-myc、c-Ha-ras和c-Ki-ras的21种组合，用1种或多种限制性内切酶Bam HI、Eco RI、Hind III、Pst I、Pvu I和Kpn I进行检测。其中16种在夏威夷人或其他种族群体中未表现出限制性片段长度多态性。在夏威夷原住民中表现出限制性片段长度多态性的4种组合在其他种族群体中表现出大小相似的限制性片段。只有在用Pst I消化c-myc的情况下，发现5名有夏威夷血统的个体具有在其他种族群体中未检测到的限制性片段长度多态性。这5个案例除了在大多数夏威夷人以及其他种族群体中总是存在的5.5kb片段外，还表现出一个13kb的c-myc片段。在夏威夷人中发现的大多数在其他种族群体中存在的限制性片段长度多态性表明，夏威夷本土基因库中原癌基因的遗传多样性并不明显低于其他种族群体。
